离子液体双水相萃取虎杖中的主要活性成分.pdfVIP

1离子液体双水相萃取虎杖中的主要活性成分 长春工业大学 冯 吉，李吉龙，马 睿，马金鹏，王家琳，孙 坤，王志兵* (长春工业大学化学与生命科学学院，吉林 长春 130012) （现代食品科技，2018,34(6):162-167） 指导教师：王志兵 副教授 中文摘要：将超声辅助离子液体/阴离子表面活性剂双水相萃取和高效液相色谱法相结合，建立一种简单、绿色、高效的分 析方法用于检测虎杖中的虎杖苷和白藜芦醇。本研究以水溶性离子液体为萃取剂，以阴离子表面活性剂为成相盐，在超声的 辅助作用下，目标分析物被萃取于离子液体中。实验结果表明，最佳提取条件为：以400 μL1-丁基-3-甲基咪唑四氟硼酸盐 为萃取剂，0.16 g 十二烷基苯磺酸钠为成相盐，样品溶液pH 7.0, 超声时间20 min，超声功率160 W。各化合物在线性范 围内都具有良好的线性关系(r＞0.9995)，日内和日间精密度分别低于 3.27 ％和6.31 ％，检出限和定量限分别为2.8～ 12.3 和9.4～41.1 ng/mL，样品加标回收率在86.88～94.13 ％之间。本研究采用离子液体代替传统的有机溶剂作为萃取剂， 集提取、分离和纯化于一体，具有操作简单、溶剂用量少、提取时间短等优点，可广泛应用于中药活性成分的提取与分析。 英文摘要: A simple, green and efficient ultrasound-assisted ionic liquid / anionic surfactant aqueous two-phase extraction coupled with high performance liquid chromatography method was developed and applied for the determination of polydatin and resveratrol in Polygonum Cuspidatum . In this study, the water-soluble ionic liquid was used as extraction solvent and the anionic surfactant was used as salt phase. Then the target analytes were extracted into the sample solution with the aid of ultrasound. After the aqueous two-phase system was formed, the target analytes were enriched in the ionic liquid. The ionic liquid phase was diluted with methanol and the resulting solution was used for chromatographic analysis. The experimental results showed that the optimized experimental conditions for the ultrasound-assisted ionic liquid aqueous two-phases extraction were: 400 μL of [C MIM][BF ] was used as extraction solvent, 4 4 0.16 g of sodium dodecylbenzenesulfonate (SDBS) was used as salt phase. The pH value of aqueous solution was 7.0. The analytes were extracted in 20 min at 160 W. The calibration curve demonstrated excellent linear relationship (r 0.9995) under the optimal conditions. The RSDs of intra-day and inter-day