《红曲发酵生产红曲色素研究》-毕业论文.docVIP

湖南化工职业技术学院普通专科毕业论文 PAGE PAGE 5 摘 要 当今社会所使用的色素原料大部分为合成色素，其副作用较大，不符合社会的需求，天然色素必将取代合成色素。而采用直接提取的方法，在生物体内提取又受到成本和气候的影响，最可靠的将是通过发酵生产。本课题主要研究红曲发酵生产红曲色素，通过对其发酵条件(碳源、氮源、起始pH、生长因子、摇瓶装液量、接种量)的研究，选择出影响较大的单因子，最后通过正交试验得到最优培养基。通过试验的出的最佳培养条件为：可溶性淀粉5.0%、蛋白胨2.0%、KH2PO4 0.5%、MgSO4·7H2O 0.05%、FeSO4·7H2O 0.001%、KCl 0.05%、VB1 0.17%、pH=5.5、接种量10%、200ml摇瓶装液100ml、温度32℃、摇床转速180r/min、发酵时间6天，其产量达到了55.4U/ml。 关键词：红曲霉；红曲色素；发酵和培养条件；单因子；正交试验 Abstract The pigment used in todays society, most raw materials for the synthesis of pigment, its side effects, do not meet the needs of society is bound to replace the synthetic pigment natural pigment. The use of direct extraction methods, extraction of biological body has been the cost and climate, the most reliable will be the production by fermentation. The main research topics Fermentation Monascus Monascus pigment, through its fermentation conditions (carbon and nitrogen sources, initial pH, growth factors, shaking bottle fluid volume, inoculum size) study, choose a greater impact on the single-factor and finally get the optimal culture medium by orthogonal test. To pass a test out of the best culture conditions as follows: soluble starch 5.0%, peptone 2.0%, KH2PO4 0.5%, MgSO4 ? 7H2O 0.05%, FeSO4 ? 7H2O 0.001%, KCl 0.05%, VB1 0.17%, pH = 5.5, inoculum 10%, 200ml bottle liquid shake 100ml, temperature of 32 ℃, shaking speed 180r/min, fermentation time of 6 days, its production reached 55.4U/ml. Keywords: Monascus ruber； Monacolin K；Condition of culture and ferment；Shan factor；Orthogonality tested TOC \o 1-3 \h \z \u 目 录 HYPERLINK \l _Toc248945778 1前言 2 HYPERLINK \l _Toc248945779 1.1 红曲霉概述 2 HYPERLINK \l _Toc248945780 1.1.1红曲的定义 2 HYPERLINK \l _Toc248945781 1.1.2红曲霉 2 HYPERLINK \l _Toc248945782 1.1.3红曲霉的分类 2 HYPERLINK \l _Toc248945783 1.1.4红曲霉的培养特征和生理生化特征 2 HYPERLINK \l _Toc248945784 1.1.5红曲霉生理活性物质 3 HYPERLINK \l _Toc248945785 1.2 MonacolinK类物质的研究进展 6 HYPERLINK \l _Toc248945786 1.2.1 MonacolinK类物质的发现 6 HYPERLINK \l _Toc248945787 1.2.2国内外已