MTRP70S6K信号通路在乳腺癌组织中的表达及临床研究.doc

MTORP70S6K信号通路在乳腺癌组织中的表达及临床研宄 滨州市中心医院甲状腺乳腺外科山东滨州251700 目的：研究mTOR/P70S6K信号通路在乳腺癌组织中的表达，探讨其在乳 腺癌发生、发展中的作用及临床意义。方法：釆用免疫组织化学技术分别检测 32例乳腺癌组织、癌旁组织以及22例正常乳腺组织中mTOR、P70S6K表达情况； 并分析乳腺癌组织中mTOR及P70S6K的表达与相关临床参数的关系.结果： mTOR在乳腺癌组织、癌旁组织及非肿瘤乳腺组织中的阳性表达率分別为59.4%、 28.1%及15.6%; P70S6K表达率分别为56.3%、31.3%及27.3%，乳腺癌组分别 与癌旁组及正常人乳腺组比较，差异均有统计学意义（P0.05）; mTOR与P70S6K 在乳腺癌组织中的表达关系呈正相关；mTOR及P70S6K在乳腺癌组织中的表达 与TNM病理分期、淋巴结转移等关系密切，而与肿瘤大小、年龄及ER状态等无 明显关系。结论：mTOR/P70S6K信号蛋白分子在乳腺癌组织中过量表达，提示 此信号通路可能在乳腺癌发生、发展过程中发挥重要作用；多项临床病理因素分 析显示mTOR及P70S6K的表达与淋巴结转移、TNM病理分期有关。 关键词：乳腺癌；mTOR； P70S6K；免疫组化技术； Expression and clinical significance of mTOR/P70S6K signaling pathway in breast carcinoma. WenXian Ding, HaiZhou Shi. Binzhou central hospital, Binzhou city, ShanDong province, China； Abstract Aim： To investigate the expression of mTOR/P70S6K signaling pathway in human breast carcinoma.Methods： Immunohistochemical method were employed to determine the expression of mTOR/P70S6K signaling pathway in cancerous and paracancerous tissuess of 32 cases and normal healthy tissues of 22 cases. The relationship between expression of the mTOR/P70S6K protein in breast carcinom tissues and related parameters was analyzed. Results： The expression level of mTOR/P70S6K signaling pathway was markedly higher in the breast carcinoma tissues than in the paracancerous tissues or normal breast tissues. There was a positive correlation between mTOR protein and P70S6K protein expression in breast carcinom tissues. The expression of mTOR and P70S6K in the breast carcinom tissues was significantly correlated with the TNM staging and lymph node metastasis，but not with tumor siz，age and the expression of ER. Conclution： The mTOR/P70S6K signaling pathway is specifically activated in breast carcinom tissues. The mTOR/P70S6K signaling pathway might play an important role in the pathogenesis of breast carcinoma. Key Words： Breast carcinoma； mTOR； P70S6K； Immunohistochemical method 乳腺癌是当前我国严重危害妇女健康的最常见的恶性肿瘤，据统计， 我国每年新增乳腺癌患者超过20万人，每年因乳腺癌死亡的人数超过4万人， 己成为