全国结核病耐药性基线调查报告.docxVIP

全国结核病耐药性基线调查报告 　　篇一：124例耐多药结核分枝杆菌基因突变特征分析 　　124例耐多药结核分枝杆菌基因突变特征分析  　　梁亚萍11 赵丽丽2 高漫1  　　摘要：目的 对临床分离的耐多药结核分枝杆菌相关基因的突变特征进行分析。方法 对124例耐多药结核分枝杆菌和50株敏感株的耐药相关基因（异烟肼inhA、 katG和oxyR-ahpC间隔区;利福平rpoB ）进行序列测定，分析其基因突变情况。结果 异烟肼耐药inhA基因突变率为%；katG基因突变率为%（87/124），主要位于315位；oxyR-ahpC间隔区突变率为% ；inhA、 katG两种基因同时突变率%，三种基因同时考虑突变率%。利福平rpoB 基因突变的检出率高达%，突变主要发生在531、526、516位点。 结论 我省耐多药菌异烟肼耐药相关基因最常见突变为katG 315、inhA C-T（-15）、axyR-ahpC间隔区(-10) C-T，利福平为rpoB531、526、516。结合MDR-TB耐药相关基因的特征分析，可以建立一种快速、准确、特异的适合于我省的检测结核耐多药性的新方法。  　　关键词：结核分枝杆菌 耐多药 基因测序 突变  　　Analysis of characteristics of genetic mutation of 124 cases of  　　multidrug-resistant mycobacterium tuberculosis  　　Liang Yaping11Zhao Lili2Gao Man1  　　[Abstract]Objective: To analyze the mutation characteristics of multidrug-resistant mycobacterium tuberculosis related :Resistant genes of 124 cases of multidrug-resistant mycobacterium tuberculosis and 50 sensitive strains (inhA,katG and oxyR-ahpC spacer of isoniazid;rpoB of rifampicin)were given sequence determination to analyze the gene :Gene mutation rate of inhA was %; gene mutation rate of KatG was % (87/124),mainly in the 315;gene mutation rate of oxyR-ahpC spacer was %;mutation rate of inhA, katG at the same time was %,mutation rate of the three genes at the same time was %.Gene mutation rate of rpoB of rifampicin was %,and mutation occurred mainly in the 531,526,516 :The most common mutation of multidrug-resistant isoniazid genes in our province were katG 315, inhA C-T (-15),oxyR-ahpC spacer (-10) C-T,and the rifampicin were rpoB531,526, analysis of combining the feature of MDR-TB resistance related gene can establish a rapid, accurate, specific and suitable new method to detect multidrug-resistant tuberculosis.  　　Key words: Mycobacterium tuberculosis ;Multidrug-resistant;Gene sequencing;Mutation  　　【中图分类号】：R52【文献标识码】：【文章编号】：  　　我国的耐药结核病流行情况较为严重，是全球27个耐药结核高负担国家之一，据全国结核病耐药基线调查结果显示[1]：我国肺结核病患者中耐多药结核（MDR-TB，指结核分枝杆菌同时对异1基金项目：陕西省卫生科研项目（)  　　作者简介：梁亚萍，女，本科，副主任检验师，主要从事结核病实验室诊断相关工作