大孔树脂法纯化玉竹总皂苷的工艺研究.PDFVIP

※工艺技术 食品科学 2012, Vol. 33, No. 18 83 王晓林，李 珍，钟方丽 * (吉林化工学院化学与制药工程学院， 吉林 吉林 132022) 目的：筛选出分离、纯化玉竹总皂苷的大孔树脂型号及工艺条件。方法：通过静态吸附与解吸实验确 定大孔树脂的型号。在静态吸附与解吸单因素试验基础上，利用四因素四水平正交试验对LSA-33 型大孔树脂纯化 玉竹总皂苷的工艺进行研究，以总皂苷吸附率、解吸率为指标，确定最佳工艺。结果：LSA-33 型树脂对玉竹总 皂苷具有较好的纯化效果，其最佳工艺为：pH6 ～7 的玉竹提取液，树脂吸附4h ，解吸液为体积分数95% 的乙醇， 解吸液用量与树脂质量比 37.5:1(mL/g) ，解吸时间为2h 时，玉竹浸膏中总皂苷的含量由未纯化前的 12.28% 提高到 41.75% 。结论：LSA-33 型大孔树脂可较好地纯化玉竹总皂苷，且操作简单、安全、成本低廉，有较高的应用 价值 。 玉竹；总皂苷；大孔吸附树脂； 纯化 　　 Purification of Total Saponins from Rhizoma Polygonati Odorati by Macroporous Resin Adsorption WANG Xiao-lin ，LI Zhen ，ZHONG Fang-li* (School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China) Abstract ：Objectives: To find out the best type of macroporous resin and determine the optimal process conditions for separation and purification of total saponins from Rhizoma Polygonati Odorati . Methods: The best type of macroporous adsorption resin for total saponins was determined by static adsorption and desorption experiments. Based on one-factor-at-a- time experiments, a four-factor, three-level orthogonal array design was employed for the optimization of four process param- eters for improved adsorption and desorption rates. Results: LSA-33 macroporous resin was found to be the best resin among 6 types of macroporous resin tested. The best purification conditions for total saponins were determined as follows: crude extract from Rhizoma Polygonati Odorati (pH 6 －7) was adsorbed for 4 h and then desorbed with 95% ethanol at a solvent-to- solid ratio of 37.5:1 (mL/g) for 2 h. As a result, the content of total saponins in the crude extract was increased to 41.75% from 12.28%. Conclusion: LSA