全基因组扩增技术及其在法医个体识别中的应用-生物通.PDFVIP

HEREDITAS (Beijing) 2010 11 , 32(11): 1119―1125 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2010.01119 全基因组扩增技术及其在法医个体识别中的应用 1 2 2 2 1 1 蔡海强 , 柳海涛 , 史斌 , 李安 , 唐文如 , 罗瑛 1. , 650224; 2. , 650224 全基因组扩增(Whole genome amplification, WGA)技术是一种对全部基因组序列进行非选择性扩增的技 术。近几年来, 对 WGA 技术扩增痕量 DNA 检材的研究日渐深入, 这些研究可望用于刑案现场采集到的痕量 DNA 样品的扩增, 为法医个体识别提供足量的DNA 模板。然而, 对实际案件中复杂检材的扩增偏差问题一直 困扰着法医工作者, 寻求一个低扩增偏差、高扩增产率的 WGA 技术是法医工作者的主要目标。文章综述了 WGA 技术在法医个体识别中的研究进展及应用前景, 为法医解决扩增偏差问题提供参考。 全基因组扩增; STR; 个体识别; 法医 Whole genome amplification and its application in forensic individual identification 1 2 2 2 1 1 CAI Hai-Qiang , LIU Hai-Tao , SHI Bin , LI An , TANG Wen-Ru , LUO Ying 1. Laboratory of Molecular Genetics of Aging and Tumor, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China; 2. Criminal investigation team, Kunming public security bureau, Kunming 650224, China Abstract: Whole genome amplification (WGA) is a technique that could non-selectively amplify the genomic DNA. In recent years, intensive studies have been made on application of WGA in trace DNA amplification, which might be applied to amplify trace DNA collected from crime scenes and provide enough DNA templates for forensic individual identification. However, the problem of amplification bias happened in the complicated test materials from real crime scenes is still trou- blesome. Development of WGA method with low amplification bias and high yield is the main target of current forensic research. Here, we reviewed the advances and