溴化呋喃酮对鳗弧菌群体感应调控行为的抑制研究.PDF

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2017, 33 4 :231-237 溴化呋喃酮对鳗弧菌群体感应调控行为的抑制研究 潘玉荣  张彩丽  朱素芹  曾名湧 （中国海洋大学食品科学与工程学院，青岛 266003） 摘 要 ： 以群体感应抑制剂— 溴化呋喃酮为研究对象，探究其对鳗弧菌群体感应相关基因和致病因子的抑制作用。利用荧 光定量PCR 检测亚抑菌浓度溴化呋喃酮对鳗弧菌vanI/R 和 luxS 基因表达量的影响，利用鞭毛染色观察溴化呋喃酮对鳗弧菌生物膜 以及鞭毛形成的作用，通过平板扩散法检测溴化呋喃酮对鳗弧菌胞外酶的作用。结果表明，溴化呋喃酮能够显著抑制鳗弧菌vanI 和luxS 基因的表达量，溴化呋喃酮能够降低鳗弧菌分泌蛋白酶和明胶酶能力，能抑制鞭毛生长和生物膜的形成并降低其运动能力。 关键词 ： 溴化呋喃酮；鳗弧菌 ；群体感应 ；鞭毛 ；胞外酶 ；生物膜 DOI ：10.13560/ki.biotech.bull.1985.2017.04.030 Inhibition of Brominated Furanone to Quorum Sensing Regulating Behaviors of Vibrio anguillarum PAN Yu-rong ZHANG Cai-li ZHU Su-qin ZENG Ming-yong （College of Food Science and Engineering ，Ocean University of China ，Qingdao 266003 ） Abstract: The present study aims to investigate the inhibition effect of a quorum sensing（QS）inhibitor，brominated furanone（BMF）， on the QS-related genes and pathogenic factor of Vibrio anguillarum. The effects of BMF on the expressions of QS-related genes（vanI/R and luxS ）weredetermined by RT-PCR at sub-minimal inhibitory concentration（MIC）. The role of BMF in the formation of biofilm and flagellum was observed by flagellum staining，and the role of BMF in the extracellular enzyme were detected by plate diffusion method. The results indicated that BMF with sub-MIC significantly suppressed the expression of vanI/R and luxS ，reduced the capacity of V. anguillarum secreting protease and gelatinase，and inhibited the growth of flagellum and formation of biofilm，i.e.，the alleviated its moving ability. : brominated furanone ； ；quorum sensing ；flagellum ；extracellular enzyme ；biofilm Key words