分子生物学Chapter 5培训.pptVIP

But how does a cell know where to cut away this short piece of pre-mRNA? It has been found that there is a polyadenylation signal on the pre-mRNA. This signal has a typical sequence of AAUAAA. This sequence gives an instruction as “to cut the mRNA about 20 nucleotides downstream, near a GU-rich sequence”. 但是，细胞是怎么知道在哪儿切去那一小段的呢？已经发现，在前体mRNA上有聚腺苷酸化信号。该信号的特征性序列是AAUAAA。该序列提示：应在下游约20个核苷酸处靠近富含GU的地方切断。 聚腺苷酸化并不是在转录产物前体mRNA本身的尾部发生的。实际上，要将前体mRNA上先切去一小段，之后poly(A)尾才被加上去 If a signal is there to tell where to cut the RNA, then who is there to respond to this signal? Actually, the polyadenylation signal