种鸡蛋传性疾病鸡白痢、禽霉形体病的新型DNA药物研究.docVIP

种鸡蛋传性疾病——鸡白痢、禽霉形体病的 新型DNA药物研究 郝钦龙伍迪肖怡陈丹 （华南农业大学动物科学学院，广东广州510642） 摘要：胚缶传染性疚病是巾母体直接传递的胚胎感染性疚病。种禽忠奋某种传染性疚痫或长 期带荫吋，会导致胚胎发生各种病理变化，甚至引起死亡。实验通过设计构建经过分子秉构 与改良的蜂毐肽类似物和鸡e-防御素多难因输卵管特异高效表达载体，经肌肉注射途径异入 鸡体内。经过重组质粒在输卵管细胞高效特异表达，使鸡体获得对沙氏菌和支原体等蛋媒疾 病的有高效抗菌性的蜂毒肽类似物和鸡(3 -防御素蛋白，有效抑杀存在于利1鸡卵巢和输卵管的 病原微生物。本实验S在发展和完善一种新型高效的预防鸡沙门氏菌感染的DNA药物。 关键词：胚蛋传染性疾病蜂毒肽鸡P-防御素沙门氏菌蛋媒疾病 Kind of egg transmission of diseases - salmonellosis, avian mycoplasma disease ofthe new DNA drugs HAO Qin-long W Di Xiao Yi Chen Dan (college of animal science，South China Agricultural University, Guangzhou 510642,China ) Abstract: Embryos infectious diseases are the direct transfer of embryos from the maternal infections. Avian infectious diseases or suffering from some kind of long-term carrier would result in a variety of pathological changes in embryogenesis, and even cause death. Experimental Design and Construction by Molecular remodeling and improvement after the melittin analogs and chicken 3 -defensin genes more highly expressed vector, attenuated intracellular bacteria in the delivery of imported chicken. After the recombinant plasmid expressed specifically in the oviduct cells efficiently, so that physical access to the sal monel la chicken and the egg-borne diseases such as mycoplasma have high antibacterial activity of bee venom peptide analogs and chicken P -defensin protein, effective suppression exists in the chicken kill ovaries and fal lopian tubes of pathogenic microorganisms. This study was designed to develop and perfect a new and efficient prevention of Salmonella infection in chicken DNA drugs Key words: Embryos infectious diseases melittin chicken 3 -defensin Salmonella egg-borne diseases 鸡通过蛋传的疾病（蛋传痫）是从感染母鸡传给新孵出后代的疾病。蛋传疾病主要有两种 传播途径：一足病原体在缶売和壳膜形成前感染卵巢滤泡，于缶形成过程屮进入缶内，如沙 门氏杆菌等；二是鸡蛋在产出时或产下后因环境卫生差，病原体污染蛋壳，例如肠道菌，特 别足沙门氏歯、大肠杆齒，时而冇绿脓杆齒、葡萄球歯及霉歯污染蛋壳等等，并通过蛋壳的 气孔进入蛋内。霉形体感染也可通过蛋传途径。由此，被传染的种蛋在孵化过程屮可能造成 死胚或臭缶，孵出的雏鸡多数为弱雏或带菌雏，在不良环境等应激因素的影响K发病或死亡 ［1.2］ O 沙门氏菌属按其生化特性分为1、II、III、IV和V五个亚属，共有2020种血淸型。我国 发现的血清型有216个。沙门氏菌的中海作