《茉莉酸甲酯与脱落酸对试管地黄块根膨大及梓醇积累的研究》-毕业论文设计.docVIP

PAGE PAGE 3 茉莉酸甲酯与脱落酸对试管地黄块根膨大及梓醇积累的研究 学院、专业 生命科学学院 生物科学 研 究 方 向 植 物 生 物 技 术 学 生 姓 名 学 号 指导教师姓名 指导教师职称 教 授 　　　 2011年5月26日 茉莉酸甲酯与脱落酸对试管地黄块根膨大及梓醇积累的研究 [摘要]：目的： 研究不同浓度的茉莉酸甲酯（MeJA）与脱落酸（ABA）对试管地黄块根膨大的影响，根据高效液相色谱仪的测定结果，分析不同浓度MeJA与ABA处理对试管地黄膨大块根中梓醇积累的影响。方法： 采用高效液相色谱法，色谱柱：Acclaim C18（4.6 mm×150 mm，5 μm）；流动相：甲醇-水（7：3）;流速：1mL/min；柱温：25℃；检测波长：210nm。 结果和结论： MeJA对试管地黄块根膨大有促进作用，ABA对地黄块根膨大有抑制作用，同时也影响试管地黄膨大过程中根的形状、根产生的数量。梓醇对照品的标准曲线：Y=40.785X，相关系数r=9.9555，试管地黄膨大块根中梓醇含量随茉莉酸甲酯浓度升高呈上升趋势。 [关键词]：试管地黄块根；茉莉酸甲酯；脱落酸；高效液相色谱；梓醇 Research on Enlargement of Tuberous Rehmannia Root And Accumulation of Catalpol to Methyl Jasmonate And Abscisic acid Qiu Qiong (School of Life Science, Huaibei Normal University) (Tutored by XUE Jian-ping) [Abstract]: Objective: According to the results of HPLC,the enlargement of root of tuberous Rehmannia were researched and the infulence of catalpol accumulation in the inflated root of tuberous Rehmannia was analyzed under different concentrations of methyl jasmonate (MeJA).Methods: By means of HPLC,HPLC column: Acclaim C18 (4.6 mm × 150 mm, 5 μm); mobile phase: methanol - water (7:3); flow rate: 1mL/min; column temperature: 25 ℃; detection wavelength : 210nm. Results and Conclusions: MeJA has accelerated the enlargement of inflated root of tuberous Rehmannia ,and also affected the shape and number of the root produced during enlargement process. The standard curve of catalpol reference substance: Y = 40.785X, the correlation coefficient r = 9.9555, the content of catalpol in the enlarged root of tuberous Rehmannia glutinosa is rising with the concentration of methyl jasmonate increasing. [Key words]: tuberous Rehmannia root; methyl jasmonate; high performance liquid chromatography; Catalpol 目录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc263078040 引言： PAGEREF _T