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分子印迹技术及其与毛细管电泳联用在复杂样品中双酚A检测的应用分析-分析化学专业论文
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Abstract
Bisphenol A (BPA), a well known environmental estrogen, has biotoxic and endocrine-disrupting effects, wildly exits in environmental, biological and food samples at a trace amount. So it is necessary to develop a fast, sensitive, high selective and cheap method for determination BPA in complex sample matrices.
Molecular imprinted technology is based on the molecular recognition theory,and its
main procedure is to synthesize molecular imprinted polymers(MIPs). Because MIPs have chemical stability and selective combination with the target analyte, they have been widely used in environmental, biological and clinical samples. Capillary electrophoresis (CE) is a noble analytical technique in analytical chemistry which has some advantages, such as highly efficient and rapid analysis.
In this thesis, a new high selective, fast and effective sample pretreatment technique-molecular imprinted solid-phase extraction (MISPE) was achieved by combination of molecular imprinted technology and solid-phase extraction (SPE). It was the first time to determine BPA in complex sample matrices by MISPE coupled with CE. The main contents in this thesis are described as follows:
In this paper, the molecular imprinted technology was systematically summed up.
Synthetic methods of molecularly imprinted polymers and application of the molecular imprinted technology in analytical chemistry were particularly outlined.
Bisphenol A imprinted polymers were synthesized by precipitation polymerization, and effects of the preparing condition, such as the ratio and volume of the porogenic solvent, the sorts of the functional monomers and the amount of template on the maximum absorption capacity and imprinted effect of the polymers were investigated. At last, 4-VP and 60ml acetonitrile were used as functional monomer and porogenic solvent, respectively, the amount of the bisphenol A was 6mmol. Under this optimal condition, regulary microspheres were obtained, the values of imprint
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