离子色谱法检测水果饮品中的蔗糖葡萄糖和果糖-中国化工仪器网.PDF

※分析检测 食品科学 2012, Vol. 33, No. 08 159 离子色谱法检测水果、饮品中的蔗糖、 葡萄糖和果糖 张 磊，周光明* ，熊建飞 (发光与实时分析教育部重点实验室，西南大学化学化工学院，重庆 4007 15) 摘 要：建立离子色谱法同时分离检测两种水果和两种饮品中的蔗糖、葡萄糖和果糖的分析方法。采用METROSEP CARB 1( 150mm ×4 .0mm) 阴离子交换分离柱和脉冲安培检测器，对淋洗分离条件进行优化。确定柱温20 ℃、淋 洗液30mmol/L NaOH 溶液，淋洗液流速1.0mL/min ，总分析时间25min 。在1.0 ～50 .0mg/L 范围内蔗糖、葡萄糖 和果糖分别呈良好线性。在优化的分离条件下，蔗糖、葡萄糖和果糖的检出限分别为0.085、0.126mg/L 和1.072mg/L (进样体积2 0 μL ，峰面积定量) 。用8.0mg/ L 蔗糖、葡萄糖和果糖的混标溶液连续5 次进样，3 种糖峰面积的相对 标准偏差分别为6 .09% 、2 .90% 和3 .03% ，保留时间的相对标准偏差分别为2 .4 9% 、1.7 8% 和1.79% 。样品测定的 回收率为83 .4 % ～12 6 .6 % 。用该方法检测了水果和饮品中的3 种糖，效果良好。同时具有操作简单，结果准确， 快速有效等优点，可为水果和饮品质量检测提供一个简单有效的方法。 关键词：离子色谱；脉冲安培检测；蔗糖；葡萄糖；果糖 Determination of Sucrose, Glucose and Fructose in Fruits and Drinks by Ion Chromatography ZHANG Lei ，ZHOU Guan g-ming* ，XIONG Jian-fei (Key Laboratory on Luminescence and Real-time Analy sis (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 4007 15, China) Abstract ：An ion chromatographic method has been developed for the determination of sucrose, glucose and fructose in two kinds of fruits and two kinds of drinks. The analytes were separated on a METROSEP CARB 1 (150 mm ×4 .0 mm) anion exchange column using 30.0 mmol/L NaOH as mobile phase at flow rate of 1.0 mL/min and detected with a pulsed amperometric detector. The total time it took to separate the sugars was 25 min. The linear ranges of sucrose, glucose and fructose were 1.0 － 50.0 mg/L. The detection limits for sucrose, glucose and fructose were 0.085, 0.126 mg/L and 1.072 mg/L (20 μL inj ection, and the quantitative analysis