干旱胁迫对玉米幼苗表达谱影响-作物遗传育种专业论文.docx

万方数据 万方数据 Abstract Drought is one of the unavoidable agricultural stresses in the world for the most serious losses among various agricultural stresses. Maize is an important food, feed and cash crops, and also is one kind of most water demanding and more sensitive to drought crops in dry land. Plants execute a number of physiological and metabolic responses to adapt to environmental stress during the long evolution process. Understanding the molecular basis of maize reaction to water stress might provide new strategies to improve the stress tolerance of agriculturally important plants. With the development of modern bioinformatics and genomics, DGE has gradually become an important tool for the studies of expression filings of genes. In this study, the differential expression of genes in the maize seedlings under drought stress were investigated by DGE and its results were verified-based on real time fluorescence quantitative PCR technology. The main results are as follows: The corn seedlings can almost withstand under 25% PEG stress, and maize seedlings maintain their growth although the their morphology indexes of maize seedlings were affected. So 20% PEG6000 was used to stress maize seeding in this study. Sequencing of hybrid maize Zheng dan 958 seedlings under 20% PEG 6000 at 0.5h、6h、48h by DGE technology, These results indicated that 683 、 2857 、 1438 genes expressed differentially in maize seedling under 20% PEG6000 stress and control at 0.5h 、 6h 、 48h and 286 、 1524 、 801 genes were up-regulated among these differentially expressed genes, and 397、13336、37genes were down-regulated expression among them. The up-regulated differentially expressed genes were less than the down-regulated ones at 0.5h, however the up-regulated differentially expressed genes were more than the down-regulated ones at 6h and 48h. The differential expression genes are the most at 6h among the 3 stressed time under PEG stress. The differentially expressed genes were categorized into t