氟保护漆、氯化镧对人工根龋的再矿化研究外科学专业论文.docx

PAGE PAGE 10 中文摘要 研究目的： 探讨单独及联合应用氟保护漆、氯化镧对人根龋的影响，为临床应用提供 实验依据和参考。 研究方法： 收集新鲜拔除没有根面龋坏的人前牙和双尖牙 50 颗，去冠，根面开窗， 脱矿形成人工龋后，随机分成五组，每组 10 颗。A 组，氟保护漆-0.1%氯化 镧处理组；B 组，氟保护漆处理组；C 组，1000mg/L 氟化钠处理组；D 组， 0.1%氯化镧处理组；E 组，去离子水处理组。C 组为阳性对照组，E 组为阴 性对照组。各组在 pH 循环模型中进行再矿化循环，整个过程在 37℃恒温 振荡中进行，每天循环 6 次，共 8 天。各组经再矿化循环处理后， 制作切 片，采用 LSCM 分析再矿化效果，SEM 观察再矿化样本病损情况。 结果： 1．通过SEM观察，A组开窗区可见大量矿物质沉积，表面平坦，致密结构 均匀，沉积物呈小斑块、条索状；C组开窗区表面矿物质沉积呈斑块、条索状结 构，较为均匀，但仍有孔隙或腔隙存在；B组介于A、C两组之间；D处理组开窗 区有矿物质沉积，表面较粗糙，沉积物呈条索状、颗粒状，结构疏松，表面有孔 隙或腔隙存在；E组开窗区表面粗糙呈凹坑样缺损，未见明显矿物质沉积。 2．通过 LSCM 观察的根龋病变样本，可以看到各处理组循环前后荧光带的 差异，经 pH 循环处理后各组所有的病损深度和荧光强度均有加深，以 E 组最为 明显, 依次是 D 组、C 组、B 组、A 组。TF 值 E 组同其他各组具有统计学差异 (P0.05 ),TF 值 C 组同其他各组具有统计学差异(P0.05)；但 AREA 值和 AF 值 A 组与 B 组无明显差异(P0.05 )。 结论： 1．氟保护漆、氯化镧单用及联合应用都有促进根龋再矿化的作用，二者联 合应用促进根龋再矿化的效果优于二者单独使用。 2．氟保护漆促进根龋再矿化的效果优于 0.1%氯化镧。 关键词 氟保护漆 氯化镧 再矿化 激光共聚焦显微镜 人工根龋 Study on re-mineralization of Fluor protector and Lanthanum chloride on the artificial root caries Abstract Objectives: To study effects of Fluor protector and Lanthanum chloride used alone and jointly to deal with human root caries to supply experiment and reference in clinical application. Methods: Fifty non-caries extracted human anterior teeth and premolars were collected with dental crown removed and fenestration district in the tooth root face. After the artificial caries were formed by de-mineralization, the tooth were randomly divided into five groups with ten teeth each. Group A: Fluor protector-0.1% Lanthanum chloride treatment; Group B: Fluor protector treatment; Group C: 1000mg / L sodium fluoride treatment; Group D: 0.1% Lanthanum chloride treatment; Group E: de-ionized water treatment. Group C is for the positive control group, and group E is the negative. Each group was carried out re-mineralization cycle, proceed in the pH cycle models at 37 degrees oscillation to circulate, six times a day, eight days together. We made manufacture chips after re-mineralization circular process and analyzed the effects of re-mineralization by