导入Bcl-2基因治疗大鼠蛛网膜下腔出血-神经外科专业论文.docx

遵义医学院硕士学位论文 遵义医学院硕士学位论文 刘胜远 万方数据 万方数据 导入 Bcl-2 基因治疗大鼠蛛网膜下腔出血 摘要 目的：通过改良方法建立大鼠蛛网膜下腔出血的动物模型，利用质粒转载导入 Bcl-2 基因，动态测量 SAH 后基底动脉内径、Bcl-2mRNA 的表达量及 HE 染色观 察血管形态的改变，探讨 Bcl-2 基因对蛛网膜下腔出血的治疗作用。 方法：采用改良枕大池二次注血法建立蛛网膜下腔出血模型，将 60 只大鼠平均 分成 A、B、C 组，A 组注入 Bcl-2 基因，B 组注入生理盐水，C 组注入空质粒， 各组分别于 SAH 后 1d、3d、5d、7d 测量 Bcl-2mRNA 的表达量，光学显微镜下观 察基底动脉的形态变化及测量其内径的大小。A 组根据大鼠断头时间分为 A1、A3、 A5 和 A7 组，同理将 B 组分为 B1、B3、B5、B7 组，C 组分为 C1、C3、C5、C7 组。 结果：大鼠模型建立后 1d 即开始出现脑血管痉挛，痉挛程度逐渐加重，于 SAH 后 7d 时痉挛最严重。基因组大鼠脑血管痉挛程度在各个时间点比生理盐水组及 空质粒组要轻，而生理盐水组与空质粒组比较无明显差异。实验中 Bcl-2mRNA 的表达量在脑血管发生痉挛开始即开始下降，于 SAH 后 7 天时最低。随着脑血管 痉挛程度的加深，Bcl-2mRNA 的表达量逐渐降低。 结论：脑血管痉挛在蛛网膜下腔出血后早期即开始发生，7天时痉挛最严重， Bcl-2mRNA表达量高时，脑血管痉挛程度要轻，Bcl-2mRNA表达量低时，脑血管痉 挛程度要重，靶向导入Bcl-2基因对治疗蛛网膜下腔出血可能有效。 关键词：蛛网膜下腔出血 Bcl-2 基因 大鼠 Targeted to insert the Bcl-2 gene to therapy subarachnoid hemorrhage of rat Abstract Objective: The purpose of this research was to establish rat models of subarachnoid hemorrhage using the improved method,to measure the inner perimeter of basilar arteries and the expression of Bcl-2mRNA and observe the morphology changes after Bcl-2 gene was transduced by plasmid,and to discuss the therapeutic effect of Bcl-2 gene to subarachnoid hemorrhage. Methods: SAH models were induced by double blood injection into cisterna magna of rats.60 SD rats were randomly divided into Group A in which Bcl-2 gene was injected , Group B in which NS was injected and Group C in which empty plasmid was injected. Bcl-2mRNA expression of each group was measured at 1d, 3d, 7d, 11d after SAH,and morphology changes of basilar arteries were observed with optical microscope.Group A was divided into four subgroups containing group A1,A3,A5 and A7.Similarly group B was divided into four subgroups containing group B1,B3,B5 and B7 which was the same as group C. Results: The phenomenon of basilar artery spasm was observed firstly at 1d after that SAH models were finished by double injections of blood into cistern magna,and the degree of spasm became severe gradually which was th