多孔陶瓷膜固定木瓜蛋白酶的研究-华南农业大学学报.PDF

第27 卷第4 期 华南农业大学学报 Vol. 27 , No.4 2∞6 年 10 月 Journal of South China Agricultural University Oct. 2∞6 多孔陶瓷膜固定木瓜蛋白酶的研究 方 焕高向阳2 张洪利2 梁建开2 林静芬2 (1广州市华琪生物科技有限公司，广东广州 510308 ;2 华南农业大学生命科学学院，广东广州 510642) 摘要:用经磷酸缓冲液处理后的多孔陶暨膜作为固定化酶的载体，采用物理吸附法固定木瓜蛋白酶-试验结果表 明，当蓓液酶质量浓度为1. 0 - 2.0 rnglrnL , pH 7.0 、固定 2 h 时，固定化酶活力最高达 11 1. 1 U/g ，活力回收达 57.9%. 固定化酶反应最适pH 为 6.0 -8. 0 ，最适温度为 60 - 70 c ,70 c下保温4h 活力仍保持在501)岛以上，半衰 期54 d. 试验证明，多孔陶壁膜吸附能力强、吸附速度快，制备的固定化木瓜蛋白酶的 pH 稳定性、热稳定性、贮藏 稳定性明显优于溶液酶. 关键词:多孔陶暨膜;木瓜蛋白酶;固定化 中图分类号:Q556.3 文献标i只码:A 文章编号:1∞1-41l X(2∞6) 04-0036-04 Preparation and Characterization of China Granule Immobility-Papain 1 2 2 FANG Huan , GAO Xiang-yang , ZHANG Hong-le , LIANG Jian-kae ,LIN Jing-fen (I Huaqi Bio-Tech Cornpany Lirnited , Guangzhou 510308 ,China; 2 College of Life Science ,South China Agric. Univ. , Guangzhou 510642 ,China) Abstract:As a new-style carrier to immobilize en町rne ， china granule was used in the immobilization of papain by physical adsorption in the experiment.咀le china granule should be treated with the phosphoric acid buffer before imrnobilization. The results of the experiment demonstrated that 出e optimum conditions for immobilization were as follows: tbe range of optimum concentration of solution papain was 1. 0 - 2. 0 mg/mL , pH value was 7. 0 and the time for immobilization was 2 h. The maximal activity of the im- mobility papain was 11 L 1 U/g and the activity recoveηof tbe immobility papain was 57. 9%.