高分辨率LAPS对溶液pH值二维分布的研究-微电子学与固体电子学专业论文.docxVIP

AbstractIn Abstract In the field of biomedicine，it7S important to learn about the distribution of the certain ions in the solution．Meanwhile，to detect different ions or molecules simultaneously is valuable as well．This dissertation describes how to measure the two—dimension distribution of pH value with high resolution LAPS(Light Addressable Potenfiometric Sensor)and how to find the best measurable conditions．By illuminating parts of the sensor surface with a light pointer,a photocurrent flows of which the amplitude depends on the local surface potential，In this way，an image of the surface potential distribution across the sensor surface can be obtained by scanning the light pointer while the photocurrent is recorded and then the two-dimension pH distribution map of the certain solution can be drawn in different gray levels． The theory analysis part mainly talks about the basic theory of LAPS, including the building of Site Binding Model，the analysis of the photo—electronics character of semiconductor and the setting up of LAPS model with EIS structure．The research on the several factors that may affect the produce of the photocurrent was also conducted． The experiment part mainly deals with the design of LAPS chip and the design of the system to realize the measure for pH value．The expected result is to tum the whole system into a practical instrument．The major work includes the design of the LAPS chip；the package of the LAPS chip and orientation of the device for high resolution measure；the debug of the laser drive circuits and the drive circuits for electromotor through DSP(TMS320C2xx)and 8031．A large number of experiments were done and relative error analysis was also fulfjlied． Keywords：EIS；LAP9 high resolution；distribution of pH value；Site Binding Mo出f． 第一章绪论第一章绪 第一章绪论 第一章绪 论 本章主要介绍了光寻址电位传感器LAPS的历史发展背景和它用于检测溶液p} 值分布的重要实用价值，以及本论文所完成的主要工作和意义。 1．1微型生化传感器的现状和发展 生物传感器在生物、医药和农业上有着广泛的应用。生物传感器是一种以生物 活性单元(如：酶、抗体、核酸、细胞等)作为敏感基元，对被分析物具有高度选 择性的现代化分析仪器。它通过各种物理、化学换能器捕捉目标物