Bacillus subtilis NKL 1的多相分类鉴定和脂肽合成基因的分子遗传学分析.docx

优秀毕业论文 精品参考文献资料 堕墅叁堂竖主堂竺堡兰 堕墅叁堂竖主堂竺堡兰 茎奎塑至 Abstract Lipopeptides biosurfaetants are microbial products．The size of lipopeptide synthetase genes is about O．7％against the whole genomic DNA size．And the genes are organized as an operon．which include a repititon gene cornS．n地genes are thought to be located at an intermediate position in a regulatory pathway which senses an environmental condition and responds by transferring a signal to the apparatus that controls the expression of genes involved in competence development and cellular differentiation．All of operon iS required for efficleat sporulation，whereas competence requires only the 5’half of genes．The operon iS controlled by two quorum sensing systems．All evidences of tanscription and regulation mechanisms of lipopeptide synthetases genes are from low-yield swains or strains that do not produce lipopeptides．LiRIe is known about high—yield strains． Bacillus subtilis NK-Lla high—yield strain，iS studied to detemine molecular genetic mechanisms of lipopeptide synthetase genes(1ps)，expecially what is transcription regulational mechanism that early competence gene comA regulates枷． T1le main research contents are listed够follows： (1)Analysised the phenotypic，genotypic and phylogenetic informations to identify the taxonomy status of the strain NK-Ll by using polyphasic taxonomy；(2)Constructed comA mutant by using knock—out technique with intergration vectors to determine the relationships of ／ps gene with comA gene；(3)Constructed two reporter gene fusions into the chromosome to research the expression model of抄gcne and coma gene；(4)To develop a strategy of constructing engineering stvdin and research the transcriptiOil mechanisms，constructed a series cloning and expression vectors and transformed them into competence NK—Ll；(5)To experiment the lligh surface active characteristics