Illumina纤微珠芯片技术1.pptVIP

Illumina BeadArray? Technology  Application ?Illumina光纤微珠芯片技术及应用?;内容 第一部分：光纤微珠芯片技术 第二部分：基因表达研究 第三部分：基因分型SNP研究 第四部分：最新技术;基因芯片的基本原理;*;基因芯片制备;Peter J. Russell, iGenetics: Copyright ? Pearson Education, Inc., publishing as Benjamin Cummings.;Peter J. Russell, iGenetics: Copyright ? Pearson Education, Inc., publishing as Benjamin Cummings.;Peter J. Russell, iGenetics: Copyright ? Pearson Education, Inc., publishing as Benjamin Cummings.;原位合成技术;点样法;Date;Date;点样技术;;1.微珠;Oligator? 合成工厂;2. 寡聚核苷酸探针;;Key Technology Elements: Bead Chemistry;;Key Technology Elements: Bead Production;acid etch;Key Elements: Random Bead Assembly;将微珠池内微珠倒入蚀刻光纤，微珠无序自组装 进入小孔，每根光纤上带一个微珠 每种微珠大约有 ~30 个重复 ;光纤束-微珠;Decoder hybridization 1; Gunderson et al. (2004) Decoding randomly ordered DNA arrays. Genome Research 14, 870-877.;Key Elements: Decoding; 每张芯片上每个微珠每个特性都被质控(100%QC) 位置和强度控制 有多少重复 小部分不合格地被捡出 是Illumina 出厂前的生产步骤 每个芯片在出厂前已通过解码和质控 解码 CD 随芯片送至用户处;Key Elements: Decoding Quality Metrics;Key Elements: Decoding Quality Metrics;BeadArray Formats;BeadArray Formats;BeadArray Formats;BeadArray Formats;BeadArray Formats;BeadStation?扫描系统;BeadArray Reader;Sentrix Bead Chip: Analytical Fluorescent Image; User Supplied Dual-block 96-well thermal cycler w/heated lid 2 plate centrifuges (pre and post-PCR) pipette ;;mVjSgOdLaI6F3B0y)v%s#pXlUiRfNcK8H5E2A+x(u$rZnWkThPeMbJ7G4C1z-wt!qYmVjRgOdL9I6F3B0y(v%s#oXlUiQfNbK8H5D2A+x*u$qZnWkShPeMaJ7G4C1z)wt!pYmVjRgOcL9I6E3B0y(v%r#oXlTiQfNbK8G5D2A-x*u$qZnVkShPdMaJ7F4C0z)ws!pYmUjRfOcL9H6E3B+y(u%r#oWlTiQeNbK8G5D1A-x*t$qZnVkSgPdMaI7F4C0z)vs!pXmUjRfOcK9H6E2B+y(u%rZoWlThQeNbJ8G4D1A-w*t$qYnVjSgPdLaI7F3C0z)vs#pXmUiRfOcK9H5E2B+x(u%rZoWkThQeMbJ8G4D1z-w*t!qYnVjSgOdLaI6F3C0y)v%s#pXlUiRfNcK8H5E2A+x(u$rZnWkThPeMbJ7G4D1z-wt!qYmVjSgOdL9I6F3B0y)v%s#oXlUiQfNcK8H5D2A+x*u$rZnWkShPeMaJ7G4C1z%s#pXlUiRfNcK9H5E2A+x(u$rZoWkThPeMbJ7G4D1z-wt!qYmVjSgOdL9I6F3B0y)v%s#oXlUiQfNcK8H5D2A+x*u$rZnWkShPeMaJ7G4C1z-wt!pYmVjRgOdL9I6E3B0y(v%s#oXlTiQfNbK8H5D2A-x*u$qZnWkShPdMaJ7F4C1z)ws!pYmUjRgOcL9H6E3B+y(v%r#oWl