CRISPRCas9技术在制备牛Myosatin基因敲除细胞株上的应用.docxVIP

优秀毕业论文 精品参考文献资料 Classified Classified Index： Code：10224 Confidential(yes／no)：No NO．：130920863 Dissertation for the Professional Master Degree Application Of CRISPR／Cas9 For Myosta tin Gene Knockout Cell Lines Candidate：Yang Yu Supervisor：Yan Yunqin Chen Hongyan Degree Category：Master of Engineering College：College of Life Science Research Field：B iology Study Mode：Bioengineering Harbin China June 2015 万方数据 目录lYIIIIi21111171111117lllll2lllll2IH2IIllilll 目录lYIIIIi21111171111117lllll2lllll2IH2IIllilll 摘要 一iI一矗_：_- ．： I 万方数据 东北农业大学工程硕士学位论文4 东北农业大学工程硕士学位论文 4讨论 ．．23 4．1 cRIsP刚Cas9技术的选择 ．23 4．2 MSTN基因靶位点的选择 ．23 4．3转染方法的选择 24 4．4细胞单克隆培养的注意事项 24 5结{仑 ．．25 致{射 一26 参考文献 ．27 攻读硕士学位期间发表的学术论文 31 万方数据 CONlENTSCONTENTS CONlENTS CONTENTS Abstract．．．．．．．． ．．．．．． ．．． ．．．．．．．．．．．．．．．． ． ．．．．．．． ．．． ．．．．．． ．．．．．．．．． ．．．．． ． I Abstract in English． 。．。，， ．．。。。 ．．．。． 。 。。．．．．．．．．。．．．． ．．．．．．。。。。。 。．．．． 。 ．．．．． ．。．。。．． 。．．．。。．Ill I Introduction．．．．．．．．．．． ．．． ． ．．．．．．．．．．．．．．． ．． ．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．1 1．1 Research progress ofCRISPR／Cas9 technology ． ． ． ．1 1．1．1 Generation and development CRISPR／Cas9 technology ． 1 1。1．2 The structure ofthe CRISPR／Cas system ． ．．．．．．． ．．．．．．．．．．．．．．．．． ．．．．．．． ．．．．．．．．．1 1．1。3 The action mechanism of type II CRISPR．／Cas systems．．．．．．．．．．．．．． ．．．．．．．．．．．． ．．．2 1．1．4 Precautions ofdesigning target sites by CRISPR／Cas9 technology ．．．．． ．．．．．．．．．．．．． 2 1．1．5 Application ofCRISPR／Cas9 technology． ．． ． ．3 i．2 The purpose and significance ofthe study ．． ．．．．．．．． ．．．．．．．．．．．．．····． ····· ········· -·”4 1．2．1 The structure and function ofMSTN gene 4 1．2．2 Research survey ofMSTN gene ．．．．．．．．．．．． ． ．． ．．．．．．． ．．．．．．． ．．．．．．-． ·-．．．-4 1。3 The purpose and significance ofthe study ． 5 2 Materials and Methods ． 6 2．1 experimental materials ． ． ． 6 2．1．1 Strain．plasmid and cell ． 6 2．1．2 Enzyme．kit and other agents ．．．．． ．．．．．．．．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．-· ·····-· 6 2．1．3 Major instrument