医用分子遗学转录和转录后修饰.pptVIP

BMB400, 3_RNA processing_04 李 希 分子医学教育部重点实验室 lixi@shmu.edu.cn Post-transcriptional Processing of RNA Making ends of RNA RNA splicing Primary Transcript Primary Transcript-the initial molecule of RNA produced--- hnRNA （heterogenous nuclear RNA ） In prokaryotes, DNA →RNA →protein in cytoplasm concurrently In eukaryotes nuclear RNA Cp RNA Types of RNA processing A) Cutting and trimming to generate ends: rRNA, tRNA and mRNA B) Covalent modification: Add a cap and a polyA tail to mRNA Add a methyl group to 2’-OH of ribose in mRNA and rRNA Extensive changes of bases in tRNA C) Splicing pre-r