郜刚分子生物学-03-染色体-2行包装和半保留复制.pptVIP

Transmission electron micrograph of human a HeLa cell, illustrating the replication bubble that characterizes DNA replication within a single replicon. A Picture Paints a Thousand Words... or more? Because a figure is as informative as 1000 words…. DNA Replication Mode （3）Semiconservative replication（半保留复制） The expected results of two generations of semiconservative replication in the Meselson-Stahl experiment.. Meselson－Stahl实验的改进之一 对15N-DNA、14N-DNA杂交分子经加热变性，对于变性前后的DNA分别进行CsCl密度梯度离心。 结果变性前的杂交分子为一条中间密度带，变性后则分为两条区带，即重密度带（15N-DNA）和低密度带（14N-DNA）。 第0代 只有15N，第一代出现杂合分子，两代后出现等量的14N和14N-15N杂合分子。纯粹