植物生长调节剂对离体香蕉叶片愈伤组织诱导的影响-广东农业科学.PDF

东农业科学 2013 年第 11 期 29 植物生长调节剂对离体香蕉叶片愈伤组织诱导的影响 1 2 1 1 朱祝英 郑锦玲 杨玉梅 刘胜辉 1.中国热带农业科学院南亚热带作物研究所袁广东 湛江 524091曰 2.广东海洋大学农学院袁广东 湛江 524088冤 摘要 以野巴西冶香蕉品种的试管芽为材料袁MS+6-BA 4.0 mg/L+NAA 0.1 mg/L+蔗糖 30 g/L+琼脂 5.0 g/L 为芽增殖培养基袁研 究 物生长调节剂对离体香蕉叶片愈伤组织诱导和增殖的影响遥 结果表明袁影响香蕉叶片愈伤组织诱导的主要因素是生长素袁而 细胞分裂素和赤霉素对其影响较小遥其中袁2,4-D 是影响诱导香蕉叶片愈伤组织发生的重要因素遥单独使用 2,4-D 就可以成功诱导 出水浸状愈伤组织袁且诱导率为 100% 曰2,4-D 浓度在 0.5~2.0 mg/L 时袁愈伤组织可在继代过程中分化成白色致密形态的愈伤组织遥 当2,4-D 和 NAA 共同作用时袁能够在继代过程中将水浸状愈伤组织继续分化成浅绿色颗粒状愈伤组织遥 关键词 香蕉叶片曰 物生长调节剂曰 愈伤组织 中图分类号 S667.9 文献标识码 A 文章编号 1004-874X 2013冤11-0029-04 Effects of plant growth regulators on the callus induction of banana leaves in Vitro 1 2 1 1 ZHU Zhu-ying , ZHENG J in-ling , YANG Y u-mei , LIU Sheng-hui (1.South Subtropical Crops Research Institute of CA TA S, Zhanj iang 524091, China; 2.College of A gronomy, G uangdong Ocean Unive rsity, Zhanj iang 524088, China) Abstract: Using in vitro suckers of 耶Brazilian 爷 banana cultivar as materials, MS +6-BA 4.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+ agar 5.0 g/L as the shoot proliferation medium, effects of plant growth regulators on callus induction from Banana leaf and its proliferation were studied. The results showed that, main factor influencing callus induction from banana leaves was auxins, while cytokinin and gibberellin had little effects. 2,4-D was the main auxin influencing the callus induction. 2,4