多功能柱净化-高效液相色谱法检测粮谷及其制品中的呕吐毒素.PDFVIP

5 3 Vol. 5 No. 3 2014 3 Journal of Food Safety and Quality Mar. , 2014 - * 鲍 蕾 , 吴振兴, 石媛媛, 静 平, 梁成珠 (, 266002) 摘 要: 目的 -, - ﹕ 方法 -(84 16, v:v), - , -结果 0.3 µg/g, 0.51.01.5 µg/g , 66.0%~98.3%, (RSDr) 1.9 %~12.7 %, HorRat 0.1~0.7 结论 , , , 关键词: ; ; ; -; Determination of deoxynivalenol in grain and grain products by multifunc- tional column clean-up and high performance liquid chromatography * BAO Lei , WU Zhen-Xing, SHI Yuan-Yuan, JING Ping, LIANG Cheng-Zhu (Technology Center of Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 26602, China) ABSTRACT: Objectives To establish a simple and rapid determination method of deoxynivalenol (DON) in grain and grain products by multifunctional column clean-up and high performance liquid chromatography ﹕ (HPLC). Methods Samples were extracted with acetonitrile-water (84 16, v:v), and the extracted liquid was purified by multifunctional columns. After drying, and re-dissolved in the mobile phase, the toxins were sub- jected to HPLC-UV analysis. The positive results were confirmed using high performance liquid chromatogra- phy-tandem mass spectrometry (HPLC-MS/MS) analysis. Results Recoveries of DON added at lev