甜瓜细菌性叶枯病胶体金免疫试纸条的研发与应用.pdfVIP

ABSTRACT APPLICATIoNoFA DEVELoPMENTAND CoLLoIDALGoLD ASSAyFoR DETECTIoNoFMUSKMELoNBACTERIALLEAF SPoT BLIGHT ABSTRACT BacterialofmelonleavescausedPseudomonas is spot by syringaepv．1achrymans distributed andinflictsdifferentof worldwidely degreesdamage．Pseudomonassyringaepv． hasbecomethreatto and cucumbermelon 1achrymans great industry． Bacterialfruitblotchisthemost melonseed—borne is important disease，thepathogen a Acidovorax is worldwide disease．MeloninfectedAcidovorax citrulli，which quarantined citrulliandPseudomonas are similartoeach syringaepv．1achrymans，the symptomsvery other．Itisdifficulttousetraditionalmethods betweenthetwo．Forthis distincting reason， theexclusionofinfestedseeds the viableand ensuring by applyingeffective，commercially convenient iscrucialforthefood detectingtechnologies industry． Theresearch was method immunizationimmunizedmice．The usingintraperitoneal cellfusion toobtain cell lines monoclonalantibodiesWaS technique hybridomaproducing sandwichmodel twodifferentmono