甘草次酸-丹参酮IIA复方脂质体处方优化及制备工艺研究.PDFVIP

网络出版时间：2013-01-09 15:36 网络出版地址：/kcms/detail/12.1108.R1536.016.html 甘草次酸-丹参酮 IIA 复方脂质体处方优化及制备工艺研究 * 林珈好，王秀丽，王玉蓉 ，何亮颖 北京中医药大学中药学院，北京 100102 摘 要：目的 研究甘草次酸-丹参酮IIA 复方脂质体（GT-Lip ）的制备工艺。方法 以大豆卵磷脂（SPC）和胆固醇（CH ） 为膜材，薄膜分散探头超声法制备甘草次酸（GA ）-丹参酮 IIA （TSN ）复方脂质体。通过单因素考察确定水合温度和探头 超声功率，正交设计法优化处方工艺；低速离心法测定脂质体中GA 与TSN 包封率，动态光散射粒径仪测定脂质体粒径与 Zeta 电位，透射电镜测定脂质体形态。结果 优化处方工艺为SPC-CH 质量比6 ∶1，SPC-TSN 物质的量之比30 ∶1，SPC-GA 物质的量之比24 ∶1，水合温度为30 ℃，探头超声条件为380 W 超声5 min；制备得到的GT-Lip 中TSN、GA 的包封率分 别为（81.50 ±0.76 ）%、（98.63 ±0.90 ）% （n ＝3 ），平均Zeta 电位为（−19±0.98 ）mV （n ＝3 ），平均粒径为（120.5±1.62） nm （n ＝3 ）。结论 所得优化工艺制备GT-Lip 稳定可行。 关键词：甘草次酸；丹参酮IIA ；复方脂质体；薄膜分散法；低速离心法 中图分类号：R283.6 文献标志码：A 文章编号：0253 - 2670(2013)07 - 0000 - 00 DOI: 10.7501/j.issn.0253-2670.2013.07.000 Preparation of glycyrrhetinic acid-tanshinone II compound liposomes A LIN Jia-hao, Wang Xiu-li, Wang Yu-rong, HE Liang-ying Beijing University of Chinese Medicine, Beijing 100102, China Abstract: Objective To study the preparation method of glycyrrhetinic acid-tanshinone IIA compound liposomes (GT-Lip). Methods Liposomes were made of cholesterol (CH) and soybean phosphatidylcholine (SPC) by film-ultrasound technique. After the exploration of single factors including hydration temperature and ultrasonic power, orthogonal design was used to select the optimum formulation. The particle size and zeta potential of liposomes were detected by Zetasizer Nano, while the encapsulation efficiencies of glycyrrhetinic acid (GA) and tanshinone IIA (TSN) were simultaneously dete