《不同浓度的A.pullulans_(ATCC9348)_多糖和ACW多糖对桦褐孔菌多酚含量及抗氧化性的影响》-毕业论文.DOCVIP

不同浓度的A.pullulans (ATCC9348) 多糖和ACW多糖对桦褐孔菌多酚和多糖含量及多酚抗氧化性的影响 摘 要：多酚的生化合成是桦褐孔菌次生代谢过程中的一个重要事件。本文以不同浓度的A.pullulans (ATCC9348) 多糖和ACW多糖为调控因子，开展了桦褐孔菌深层发酵过程中酚类化合物及胞外多糖积累的研究。不同浓度的支链胞酶、短梗菌酶多糖的加入促进了酚类化合物的积累，特别是EPS of ACW-45ug/L的加入明显提升了酚类化合物的积累，在第10天胞内外酚类化合物积累最多，分别为71.8785±1.17009mg/g和182.1643±2.00726 mg/L。EPS of A.pullulans-45ug/L的加入促进了桦褐孔菌胞外多糖含量的提升，在第10天达到最大值4.2517±0.07667g/L 。同时不同浓度的A.pullulans (ATCC9348) 多糖和ACW多糖的加入显著地提高了酚类化合物清除自由基的能力。 Abstract: Synthesis of Polyphenol are an important event in the process of Inonotus obliquus secondary metabolites . In this paper, different concentrations of EPS of ACW and EPS of A.pullulans as a control factor, carried out to study the accumulation of phenolic compounds and extracellular polysaccharide in Inonotus obliquus submerged fermentation process. different concentrations of EPS of ACW and EPS of A.pullulans r added as a regulatory factor could improve the accumulation of phenolic compounds significantly., In particular the EPS of ACW-45ug / L added could enhance the accumulation of phenolic compounds significantly, In the first 10 days maximum accumulation of phenolic compounds inside and outside of the cell, were 71.8785 ± 1.17009mg / g and 182.1643 ± 2.00726 mg / L. EPS of A.pullulans-45ug / L promoted the addition of Inonotus obliquus exopolysaccharides content in the first 10 days, maximum was 4.2517 ± 0.07667g / L. the total phenols increased Maximum 922.4246054mg / L. At the same time, different concentrations of EPS of ACW and EPS of A.pullulans r added could improve the capacity of scavenging free radicals of the phenolic compounds significantly. 关键词：桦褐孔菌，次生代谢，深层发酵，酚类化合物，调控因子 Keywords: Inonotus obliquus, secondary metabolites, submerged fermentation, phenolic compounds, control factor 桦褐孔菌(Inonotus obliquus)属担子菌亚门、层菌纲, 非褐菌目、多孔菌科、褐卧孔菌属。分布于北美、芬兰、波兰、俄罗斯及我国黑龙江、吉林等地区, 寄生于桦树、榆树、赤杨等的树皮下或活立木的树皮下或砍代后树木的枯干上[1]。该菌在俄罗斯已有一百多年的应用历史, 是具有显著的药理活性而对人体没有任何副作用的一种药用真菌[2]。研究表明：酚类化合物是桦褐孔菌主要活性成分，具有抗氧化、抗肿瘤[3] 、抗病毒、增强机体免疫[4]、益肝护肾、抑菌、抗艾滋病[5]等作用。因此