利用微悬臂梁传感对瘦肉精进行非标记检测.PDF

24 2 2009 4 Journal ofM ed ical Biom echanics, V ol. 24 N o. 2, A pr. 2009 89 : 1004-7220( 2009) 02-0089-05 # # 1 1 1 2 2 1 黄 渊, 薛长国, 张青川, 谭伟明 , 王保民 , 伍小平 ( 1. , 230027; 2. , 100094) : ,, ( ELISA) 1L g/L, , 1mg/L, , : ; ; ; : R155. 5 : A Labe-l free detection of clenbuterol based onm icrocantile er 1 1 1 2 2 HUANG Yuan, XUE Chang-guo, ZHANG Q ing-chuan, TANW ei-ming, WANG Bao-min, 1 WU Xiao-ping. (1.K ey Laboratory of M echanicalB ehavior andD esign of M aterial of ChineseA cademy of Sciences, Un i- versity of Science and echnology of China, H ef ei 230027, Ch ina; 2. College of Agronomy andB iotechnology, China Agri- cultural University, B eij ing 100094, China) Abstract: Objecti e A novel technology ofmicrocantilever sensorplatform for labe-l freedetection of clenbuterol antigen- antibody specificitybinding is reported. M ethod Thiolated clenbuterolantibodywas mi mobilized on thegold-coated sideof themicrocantileverby self-assembledmonolayer technique. Consequently the deflections of them icrocantilever correspond- ing to the process of injecting different concentration of clenbuterol antigenwere rea-l tmi emonitored by optical lever tech- nique. The activity of thiolated clenbuterolantibody and the clenbuterol antigen-antibodybinding on thegold-coated side of themicrocantileverwereconfirmed by anEnzyme-Linked ImmunosorbentAssay ( ELISA). R esult The sensitivi