OPGRANKLRANK系统参与牙槽骨吸收及重建过程作用的初探.doc

OPG/RANKL/RANK系统参与牙槽骨吸收及重建过程作用的初探 陈莉丽，黄 玫，雷利红，吴燕岷（ 310009杭州，浙江大学医学院附属第二医院口腔内科） [摘要]目的 初步探讨护骨素OPG、核因子κB受体活化因子配体RANKL、核因子κB活化因子RANK在牙周炎牙槽骨吸收及骨重建过程中的作用。方法 对小鼠单核巨噬细胞白血病细胞株RAW264.7实验组进行体外诱导培养，对照组为完全培养基培养；小鼠成骨样细胞株MC3T3-E1实验组采用前期实验收集的骨吸收上清处理，对照组采用完全培养基培养；以免疫荧光等方法检测细胞OPG、RANKL、RANK的表达。30只8周龄雄性SD大鼠建立实验性牙槽骨吸收模型，研究其吸收重建规律。以免疫组化S-P法检测OPG、RANKL、RANK的表达情况。结果 实验组MC3T3-E细胞经骨吸收上清处理7天后，OPG蛋白平均荧光强度为29.636±5.652，对照组为15.568±1.229，表达显著上调（P0.01）；但实验组RANKL蛋白平均荧光强度：6.806±1.738，对照组为18.082±2.732表达被显著抑制（P0.01），OPG/RANKL比值在上清处理后高于对照组（P0.05）。采用商品化大肠杆菌内毒素E-LPS注射法成功制备大鼠牙槽骨吸收模型。在牙槽骨吸收区域OPG水平较无骨吸收区域有所降低，RANKL的表达则相反。结论 OPG、RANKL、RANK参与了大鼠实验性骨吸收活动；破骨细胞骨吸收上清可能通过改变OPG与RANKL的比值，从而影响骨重建中骨形成与骨吸收的动态平衡。 [关键词]成骨细胞；破骨细胞；护骨素； 核因子κB受体活化因子配体；核因子κB活化因子；牙周炎动物模型 [中图法分类号] Role of The primary study of OPG/RANKL/RANK system during the alveolar bone resorption and remodeling ChenHEN Lili，, HuangUANG Mei，, LeiEI Lihong，, WUu Yanming （(Department of Oral Medicine，, the Second Affiliated Hospital，, School of Medicine，, Zhejiang University，, Hangzhou, Zhejiang Province, 310009, China） [Abstract] Objective To investigateexplore the potential role effect of a system containing osteoprotegerin (OPG), receptor activator of NF-κB ligand (RANKL), and receptor activator of nuclear factor-kappa B (RANK), also as, OPG/RANKL/RANK system in the resorption and remodeling mechanisms of alveolar bone. Methods Mouse RAW264.7 cell line was induced to osteoclasts in vitro， , and mouse MC3T3-E1 cell line was treated with bone resorption supernatant；. tThe control groupcells was cultured with regular medium；. tThe expression levels of OPG，, RANKL， and RANK expression level in the cells was detected viaby immunofluorescence methodassay. Thirty 8-weeks -old male SD rats were used to establish the alveolar bone resorption model with the injection of E-LPS. Immunohistochemical S-P method was used to investigate the expression of OPG，, RANKL， and RANK in the animal model. Results The expression of OPG in MC3T3-E1 cells was up-regulated aft