SDF-1对MSC向血管内皮细胞分化的影响.doc

SDF-1对MSC向血管内皮细胞分化的影响 冯柳，迟路湘 (400038 重庆，第三军医大学西南医院心血管内科) [摘要] 目的 探究基质细胞衍生因子-1（Stromal cell-derived factor-1，SDF-1 ）对人骨髓间充质干细胞(Bone marrow mesenchymal stem cells, BMSC)向血管内皮细胞（Vascular endothelial cell, VEC）分化的作用。方法 BMSC分为空白组（A组）；实验组：10、25、50ng/mlSDF-1组（B1、B2、B3组），25ng/mlVEGF+25ng/mlSDF-1组(B4组)；对照组：25 、50ng/ml VEGF组（C1、C2组），在添加相应细胞因子的无血清培养基中诱导12天。免疫组织化学检测诱导后BMSC的血小板内皮细胞粘附分子（Platelet endothelial cell adhesion molecules,PECAM-1即CD31）、血管性血友病因子(Von Willebrand Factor, vWF)表达情况。实时荧光定量PCR（Q-PCR）检测vWF、PECAM-1、血管细胞粘附分子-1（Vascular cell adhesion molecule,VCAM-1）、血管内皮生长因子受体-2（Vascular endothelial growth factor receptor-2,KDR）、血管内皮钙粘蛋白(VE-cadherin, VE)的表达情况。结果 B4组KDR、VE、vWF、CD31、VCAM-1表达及CD31、vWF阳性率分别较B1、B2、B3组显著升高（p＜0.0001）；B4组VE、vWF表达较C1组显著升高（p＜0.0001）且vWF阳性率较C1组也显著升高（p＜0.001）；B4组KDR、CD31、VCAM-1表达及CD31阳性率较C2组显著升高（p＜0.001）且CD31阳性率较C2组也显著升高（p＜0.01）。结论 SDF-1对MSC向EC分化起促进作用，联合使用SDF-1和VEGF可以更好的促进MSC向EC分化。 [关键词] 基质细胞衍生因子-1；血管内皮生长因子A-165；骨髓间充质干细胞；血管内皮细胞 [中图分类号] R322 12; R329 21; R329 24 Effect of MSC differentiation into VEC by SDF-1 induced Feng Liu1, Chi Lu Xiang2 (1, 2 Department of Cardiology, Southwest Hospital, Third Military Medical University, Chongqing, 400038) [Abstract] Objective To study the Effect of SDF-1 inducing human BMSC differentiation into VEC. Methods Group of Experiments: Blank group(A); Experimental Group: group of 10/25/50 ng/mL SDF-1(B1/B2/B3), group of 25ng/ml VEGF+25ng/ml SDF-1 (B4)；Control group: group of 25/50ng/ml VEGF (C1/ C2). BMSC of different group were cultured in serum free medium with the corresponding cytokines for 12 days. Immunohistochemistry assays detected positive rate of CD31 and vWF on BMSC. Q-PCR measured the mRNA levels of vWF, VCAM-1, KDR, VE and CD31. Results The mRNA levels of KDR, VE, vWF, CD31 and VCAM-1and positive rate of CD31 and vWF of B4 are significantly higher than B1, B2, B3(p 0.001); The mRNA levels of VE and vWF of B4 are higher than C1 (p 0.0001), positive rate of vWF of B4 is significantly higher than C1 (p 0.00