腺相关病毒介导PSA基因转染DC实验研究.pptVIP

前列腺癌细胞大量表达PSA PSA是前列腺细胞内的一种特异性蛋白； ——前列腺癌标志物． 选择PSA作为前列腺癌目标抗原． Gene delivery loading The gene can produce a constant and large amount of protein（ＰＳＡ）!!!!! 研究结论 成功构建了AAV/PSA质粒，其能够高效转染DC； AAV/PSA基因成功整合入DC染色体内； DC内成功表达PSA蛋白； AAV/PSA转染DC后，仅1周即可诱导得到大量、高活性的CTL； 所获得CTL对PSA阳性的前列腺癌细胞具有特异、高效的细胞毒杀伤效应。 * * 腺相关病毒介导PSA基因 转染DC的实验研究 　　尤长宣 罗荣城 梁卫江　 　南方医科大学南方医院肿瘤中心 The Oncology Department of Nanfang Hospital, the Southern Medical University, Guangzhou Adaptive and Innate Immune Systems Skin Mucous Membranes rapidly regenerating surfaces, peristaltic movement, mucociliary escalator, vomiting, flow of urine/tears, coughing Cellular and Humoral Defenses lysozyme, sebaceous/mucous secretions, stomach acid, commensal organisms, complement proteins, phagocytosis, natural killer cells Invasion infection Barriers Innate immunity Adaptive immunity Inflammation Cellular and Humoral Defenses Antibodies, cytokines, helper T cells, cytotoxic T cells（CTL） MK-0069-01 Ag /MHC Class II Ag/ MHC Class I CD4 Activation Cytokines CD8 TARGET LYSIS Activation （CTL） Dendritic cell - MHC Class I restricted - antigen specific Antigen presentation Tumor antigen Antigen positive tumor cell Dendritic cells express a variety of cell surface molecules which play key roles in their interactions with T cells. The MHC molecules present antigenic fragments (peptides) to T cells in a form that can be recognized in a highly specific fashion by the appropriate T cell receptor, which is unique for every T cell and every antigen. The costimulatory molecules (CD80 and CD86) provide a second-signal that amplifies the antigen recognition process. Accessory molecules like ICAMs and LFAs enhance the physical interaction between T cells and DCs. Dendritic Cell Properties Loading dendritic cells with the antigen １．Protein/peptide loading ２．Gene delivery loading Disadvantages of Protein/peptide loading T1/2 of proteins/peptides is very short. All are rapidly lost. 2) Most protocols fo