《适冷α-淀粉酶菌株培养基研究》-毕业论文设计（学术）.docVIP

适冷淀粉酶发酵培养基研究 大连大学 DALIAN UNIVERSITY DALIAN UNIVERSITY 2013届毕业论文（设计） 题目名称：适冷α-淀粉酶菌株培养基研究 所在学院： 生命科学与技术学院 专业（班级）： 09级生物工程2班 学生姓名： 周新尚 指导教师： 窦少华 评阅人： 院 长 ： PAGE \* MERGEFORMAT PAGE \* MERGEFORMAT 1 摘 要 适冷淀粉酶是一种重要的适冷酶类，主要来自于适冷微生物。本研究从大连大黑山筛选出1株适冷淀粉酶高产菌株，通过菌株的形态特征鉴定确定其为微小杆菌属，为革兰氏阳性菌。对淀粉具有专一分解能力，命名为菌株D2。 对菌株D2的发酵产酶培养基进行单因素实验研究，在单因素试验的基础上，采用Plackett-Burman(P-B)设计和响应面试验设计(RSM)对产酶进行优化。影响菌株D2产酶的主要因素是可溶性淀粉、牛肉膏和氯化钠的添加量，可溶性淀粉42.32g/L ,牛肉膏15.35g/L，氯化钠15.66g/L时最利于适冷淀粉酶的产生，酶活能达到116.05U/mL，比优化之前提高了247.86％。 关键词：适冷微生物；适冷淀粉酶；发酵培养基；发酵培养基优化  Abstract Cold-adapted amylase is an important cold-adapted enzymes, mainly from the cold-adapted microorganisms. This study Dalian Montenegro screened a cold-adapted amylase producing strain, strain through morphological characterization identified it as tiny spp, Gram-positive bacteria. Ability to decompose starch with specific named strain D2. Against strains of D2 Fermentation medium for univariate experimental study, based on a single factor experiments, using Plackett-Burman (PB) design and response surface analysis (RSM) on enzyme production optimization. D2 strain effect is a major factor enzyme soluble starch, beef extract, and the added amount of sodium chloride, soluble starch, 42.32g / L, beef extract 15.35g / L, NaCl 15.66g / L the most favorable cold-adapted amylase production, enzyme activity can reach 116.05U/mL, than before optimization increased 247.86%. Keywords: cold-adapted microorganisms; cold-adapted amylase; fermentation medium; fermentation medium optimization 目录 TOC \o 1-3 \h \u HYPERLINK \l _Toc23093 绪 论 PAGEREF _Toc23093 5 HYPERLINK \l _Toc22502 1.本文立题意义 PAGEREF _Toc22502 5 HYPERLINK \l _Toc26901 2.1 极端微生物 PAGEREF _Toc26901 5 HYPERLINK \l _Toc11189 2.1.2 适冷酶简介 PAGEREF _Toc