《膜蛋白的结构与功能(三)》-课件设计（公开）.pptVIP

Bacteriorhodopsin Proton pathway Simulations show Schiff base proton rotates 180o upon K-to-L transition. - Helix C bend changes its electrostatic environment. L-state shows two plausible proton transfer pathways. Direct. Via Thr89. Multiple pathways plausible. Bacteriorhodopsin Mechanistic Conclusions Bacteriorhodopsin best characterised membrane protein. Why does the primary proton transfer take ~ 40 msec? Facilitated by a transient bend of an a-helix. How are the pKas of key groups perturbed? Retinal isomerisation breaks a H-bond from the SB to a water molecule. SB reorientates int