麻黄碱对缺氧缺血新生大鼠海马神经细胞的保护作用.doc

文章编号：200909017 论著 PAGE PAGE 5 文章编号：200909017 麻黄碱对缺氧缺血新生大鼠海马神经细胞的保护作用 许 蓓1，肖 农1，张晓萍2 （重庆医科大学：1附属儿童医院康复中心，2儿科研究所，重庆 400014） 摘 要： 目的 观察麻黄碱（ephedrine,EPH）对大鼠缺氧缺血性脑损伤（Hypoxic-ischemic brain damage,HIBD）海马神经细胞凋亡及远期学习记忆能力的影响。方法 新生SD大鼠（P7）随机分为麻黄碱组(EPH组)，对照组（Con组）和假手术组（Sham组）。分别于HIBD后6 h， 12 h， 1 d， 3 d， 7 d采用免疫组织化学检测各组大鼠Bcl-2 蛋白和 Bax蛋白在海马区的表达，HIBD后4 w Morris水迷宫测试其学习记忆能力。结果 EPH组Bcl-2表达较Con组显著增高，1 d时达到高峰，3 d后逐渐下降；Bax蛋白表达较Con组明显降低，1 d时最低；各组平均潜伏时间均逐渐缩短，EPH组3～5 d时平均潜伏时间较Con组明显缩短。EPH组穿越平台次数及在目标象限游泳距离与总距离百分比均高于Con组。结论 麻黄碱可使新生大鼠HIBD后海马Bcl-2蛋白表达增加和Bax蛋白表达减少，并能提高远期学习记忆能力。 关键词：缺氧缺血性脑损伤；麻黄碱；Bcl-2；Bax；水迷宫 中图法分类号：R364.4 R722.1 文献标识码：A Neuroprotective effects of ephedrine after hypoxic-ischemic brain injury in neonatal rats Bei Xu 1， Nong Xiao 1， XiaoPing Zhang 2（1.Department of Rehabilitation Center，Children’s Hospital of Chongqing Medical University，Chongqing 400014，China；2.Pediatric Research Institute，Childrens Hospital of Chongqing Medical University，Chongqing 400014，China） Abstract Objective To explore the effects of ephedrine on hippocampal cell apoptosis? and behavioral performance after hypoxia-ischemia brain injury in neonatal rats. Methods All rats were randomly divided into 3 groups,i.e. ephedrine treated group， control group， and sham group. At each time interval of 6h， 12h，1d， 3d， 7d after hypoxia， expression of Bcl-2 and Bax were detected in the hippocampal region by immunohistochemistry. At 4 weeks after surgery， behavioral changes in the remaining rats were tested by Morris water maze. Results (1)Compared with control group,the expression of Bcl-2 in the ephedrine treated group was significantly increased after hypoxic-ischemic, peaked at 1 d and decreased after 3 days?. And the expression of Bax in the ephedrine treated group was decreased 1 d after hypoxic-ischemic; (2) The average ti