结直肠癌组织中MAC30的表达及其临床意义.docVIP

基金项目：国家自然科学基金资助项目 Supported by the National Natural Science Foundation of China 作者简介：赵增仁，男，副教授，博士，主要从事消化道肿瘤的研究，发表论文20余篇。 E-mail： zzr-doctor@163.com 通讯作者：张志勇， E-mail： HYPERLINK mailto:zhiyongzhang1@ zhiyongzhang1@ 收稿日期： 结直肠癌组织中 MAC30 的表达及其临床意义 赵增仁1，张丽静1，张志勇2，高艳敏2，张学明1，胡月明2，李芳1，孙晓峰1 （河北医科大学 1 第一医院普外科，石家庄 050031；2 唐山工人医院病理科，唐山 063000） 摘要： 目的 探讨脑膜瘤相关蛋白（Meningioma-associated protein, MAC30）mRNA在结直肠癌中的表达及其临床意义。方法 采用RT-PCR法对50例结直肠癌原发肿瘤组织、瘤旁正常粘膜组织、近端及远端切缘正常粘膜组织中MAC30、Particularly interesting new cysteine- histidine rich protein (PINCH)、核孔蛋白88 (Nucleoporin 88, Nup88) mRNA 的表达进行检测和分析。结果 结直肠癌组织中MAC30 mRNA的表达 (0.57±0.36) 明显高于癌旁无瘤粘膜 (0.35±0.25)、远端切缘粘膜 (0.35±0.28) 以及近端切缘粘膜 (0.34±0.25) (P ＜0.05)。在粘液癌/印戒细胞癌中，MAC30 mRNA的表达较非粘液性腺癌的表达水平明显增高(P = 0.009）；与PINCH mRNA、Nup88 mRNA的表达呈正相关(P 均＜0.0001)。MAC30 mRNA的表达与患者年龄、性别、肿瘤位置、分化程度及浸润深度无关 (P ＞0.05)。结论 MAC30 mRNA的表达与结直肠癌的发生发展有关，可作为评估结直肠癌预后的一个参考指标。 关键词：结直肠肿瘤；MAC30；PINCH；Nup88 中图分类号：R656.9 文献标识码：A Expression of MAC30 mRNA in colorectal carcinoma and its clinical significance ZHAO Zeng-ren1, ZHANG Li-jing1, ZHANG Zhi-yong2, GAO Yan-min2,ZHANG Xue-ming1, HU Yue-ming2, LI Fang1, SUN Xiao-feng1. ( 1 Department of General Surgury,the First Hospital ,Hebei Medical University, Shijiazhuang 050031, China; 2 Department of Pathology,Tangshan Gongren Hospital, Hebei Medical University, Tangshan 063000, China) Abstract： Objective To investigate the expression of MAC30 mRNA in colorectal carcinomas as well as the relationship between the expression and clinicopathological features. Methods MAC30, PINCH and Nup88 mRNA expression was examined by RT-PCR in 50 primary carcinomas, matched normal mucosa samples of two surgery marginal mucosa and non-cancerous mucosa which were 2 cm away from tumors. Results MAC30 mRNA expression was increased from proximate normal mucosa (0.34±0.25), non-cancerous mucosa(0.35±0.25) and distant normal mucosa (0.35±0.28) to primary tumor (0.57±0.36, all P 0.05). MAC30 mRNA