四物消风颗粒对变应性接触性皮炎小鼠胸腺,脾脏及其T,B淋巴细胞增殖的影响.docVIP

四物消风颗粒对变应性接触性皮炎小鼠胸腺、脾脏及其T、B淋巴细胞增殖的影响 王辰允1，孙学刚2，刁建新2，赵晓山3 (100853 北京，. 中国人民解放军总医院科1？1；，北京 100853，2.510515 广州， 南方医科大学：中医药学院中医证候实验室2，，广东广州 510515，3. 南方医科大学南方医院中医科3，广东广州 510515） [摘 要]： 目的 探讨中药四物消风颗粒对变应性接触性皮炎(英文全写？ACD)小鼠免疫器官胸腺、脾脏及其T、B淋巴细胞增殖的影响。方法 将60只小鼠随机分为6组，分别为：正常对照组、ACD模型对照组、氢化可的松阳性对照组及四物消风颗粒高、中、低剂量组，每组10只。观测各组小鼠耳肿胀程度、胸腺指数、脾脏指数以及丝裂原诱导的脾T、B淋巴细胞的增殖反应。结果 四物消风颗粒高、中、低剂量组均能不同程度地抑制ACD模型小鼠的炎症程度（P＜0.05），显著降低ACD小鼠胸腺指数和脾脏指数（P＜0.05），抑制丝裂原诱导的脾脏T、B淋巴细胞增殖反应，存在明显的量效关系（P＜0.05）。结论 四物消风颗粒防治小鼠ACD的药理机制与其调节T、B淋巴细胞功能作用有关。 [关键词]： 四物消风颗粒；变应性接触性；皮炎；免疫器官；淋巴细胞 [中图法分类号] [文献标志码] A Effects of Siwuxiaofeng granulas on the thoracic glands, spleen and its lymphocytes proliferation of ACD mice WANG Chen-yun1, SUN Xue-gang2 ,DIAO Jian-xin2, ZHAO Xiao-shan3王辰允 王辰允，女，博士，药师，研究方向：新型药物递送系统，Tel：86-10E-mail：cynthia_yun@。 [通讯信作者]： 赵晓山，男，博士，副主任医师； 研究方向：中医药防治亚健康；，电话：Tel： （020）E-mail： zhaoxs2004@ (1.General Hospital of PLA, Beijing, 100853, China; 2.Laboratory of TCM Syndrome, the Southern Medical University, Guangdong, 510515, China; 3.Department of TCM, Nanfang Hospital, the Southern Medical University, Guangdong, 510515, China) [Abstract]: Objective To observe effects of Siwuxiaofeng granulas on the thoracic glands, spleen and its lymphocytes proliferation of allergic contact dermatitis(ACD) mice. Methods The 60 mice were random divided into six groups: the normal control group, the ACD model group, hydrocortisone group and the Weikang-granule high, middle, low dose groups(n=10). At the end of the experiment, the swelling of auricle of mice were determined. To measure the indexes of spleen and thoracic glands of mice and to assay lymphocytes proliferation by CCK-8. Results Comparing with model group, Weikang-granule high, middle, low dose groups could remarkably decrease the swelling of auricle(P0.05), apparently reduce the indexes of pleen and thoracic glands(P0.05), markedly suppress the T and B lymphocytes proliferation induced by mitogen, an