慢性脑缺血大鼠海马Notch1表达的动态变化.docVIP

慢性脑缺血大鼠海马Notch1表达的动态变化与进行性认知功能障碍的关系[ [基金项目] 国家自然科学基金 资助项目 [通信作者] 张博爱，电话：（0371 E-mail:zhangboaidoctor@hotmail. com 姬亚杰，张博爱，刘荣丽 ，李星 ，贾贺， 张小敏，刘宇 （450052郑州，郑州大学第一附属医院神经内科） 摘要 目的 观察慢性脑缺血大鼠海马Notch信号通路的表达变化，初步探讨该通路在慢性脑缺血后对认知功能的调控作用。 方法 SD大鼠，随机如何随机？请指明具体的随机方法。如果是随便分的，则请删除随机二字采用随机数字表法分为假手术组、缺血8周组、缺血10周组、缺血12周组，每组10只。采用双侧颈总动脉永久性结扎方法（2-VO）制备慢性脑缺血大鼠模型，Morris水迷宫法测定各组大鼠学习记忆功能，应用免疫组织化学染色及Western blot 法测定大鼠手术后各时间点海马Notch1的表达水平。 结果 假手术组的潜伏期为（32.50±9.70）s，缺血8周组、缺血10周组、缺血12周组的潜伏期分别为（51.00±11.16）、（63.50±14.11）、（77.90±8.41）s, 缺血后各组大鼠的学习记忆能力较假手术组显著下降(P0.01)；缺血8周组Notch1表达的阳性细胞数（46.60±14.50）较假手术组（64.10±14.66）明显降低，缺血后10周阳性细胞数（24.70±9.38）进一步降低，缺血后12周阳性细胞数（53.20±14.22）又有升高趋势，达缺血8周组水平（P＞0.05）。Western blot检测结果与免疫组化检测结果一致缺血8周组和缺血10周组Notch1的表达较假手术组呈进行性下降，缺血12周组Notch1的表达较缺血10周组显著增高,较缺血8周组无统计学意义。 结论 在大鼠慢性脑缺血模型中，Notch信号通路呈现动态表达变化，提示该通路可能参与了缺血后认知功能障碍的调节。 如何随机？请指明具体的随机方法。如果是随便分的，则请删除随机二字 [关键词] ：慢性脑缺血；Notch1；认知功能 中图法分类号：R741.02 Relationship between expression of Notch1 and cognitive function in rat hippocampus after chronic cerebral ischemia Ji Yajie, Zhang Boai, Liu Rongli, Li Xing, Jia He, Zhang Xiaomin ,Liu Yu (Department of Neurology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China) [Abstract] Objective To detect the expression patterns of Notch1in the hippocampus of chronic cerebral ischemia and investigate whether the Notch pathway is involved in the regulation of cognitive impairment in the hippocampus under ischemic conditions. Methods Chronic cerebral ischemia models were established by permanent occlusion of bilateral common carotid arteries (2-VO) in SD rats. The rats were assigned to 4 groups: those with chronic cerebral ischemia for 8 weeks, 10 weeks, 12weeks, and those given sham operations. The spatial memory ability of the rats was tested with Morris water maze. Immunohistochemistry and western blot were used to detect the protein level of Notch1. Results The intelligence of rats 8, 10, and12 weeks after the operation was impa