大鼠肺鳞癌癌变过程中细胞周期调控蛋白的动态表达.docVIP

大鼠肺鳞癌癌变过程中细胞周期调控蛋白的动态表达基金项目： 基金项目：国家自然科学基金面上项目 Supported by the National Natural Science Foundation of China 作者简介：刘文斌，男，湖南省华容县人，博士研究生，主要从事肺癌分子机制方面的研究。电话：(023E-mail: wenbinliutmmu@ 通信作者：曹 佳，电话：(023E-mail: HYPERLINK mailto:caojia@ caojia@ 收稿日期：2008-08-12；修回日期：2008-12-20 刘文斌，刘晋袆，周紫垣，周燕虹，敖 琳，崔志鸿，曹 佳 (第三军医大学军事预防医学院军事毒理学教研室， 重庆 400038) 摘 要：目的　探讨化合物诱导的大鼠肺鳞癌癌变各阶段细胞周期调控蛋白p16、p27和p53水平动态变化趋势、相互关系及其在癌变过程中的作用。 方法　支气管灌注3-甲基胆蒽(MCA)及二乙基亚硝胺(DEN)碘油溶液诱发Wistar大鼠肺鳞癌。采用免疫组化S-P法检测p16、p27和p53蛋白在大鼠肺鳞癌癌变各阶段的表达水平，采用Western blot检测鳞癌、癌旁和正常组织中各蛋白表达水平。 结果　共获取癌变各阶段标本154例，其中增生25例，鳞状化生27例，不典型增生37例，原位癌30例，浸润癌25例。p16和p27蛋白表达的阳性率随癌变进展而降低，p53蛋白表达的阳性率随癌变进展而升高。p16与p27蛋白表达呈正相关(P 0.01)，p16和p27蛋白表达与p53蛋白表达呈负相关(P 0.05)。鳞癌组织中p16与p27蛋白低表达，p53蛋白高表达，与正常组织或癌旁组织有显著性差异(P 0.01)。 结论　在癌变过程中细胞周期调控蛋白p16和p27 表达下调，p53蛋白表达逐渐增强，可能与大鼠肺鳞癌的发生、发展密切相关。 关键词：肺肿瘤；癌变；p16；p27；p53 中图法分类号：R734.2 文献标识码：A Dynamic Eexpression of Ccell Ccycle Rregulation Rrelated Pprotein during Ccarcinogenesis of Llung Ssquamous Ccell Ccarcinoma in Rrats LIU Wen-bin, LIU Jin-yi, ZHOU Zi-yuan, ZHOU Yan-hong, AO Lin, CUI Zhi-hong, CAO Jia (Department of Military Hygienic Toxicology, College of Military Preventive Medicine College, Third Military Medical University, Chongqing 400038，, China) Abstract: Objective To investigate dynamic expressions and correlation of cell cycle regulation related proteins, pP16, pP27 and pP53 during the carcinogenesis of chemically-induced lung squamous cell carcinoma in Wistar rats. Methods Squamous cell carcinoma of lung was induced by left intra-bronchial instillating with 3-methylcholanthrene (MCA) and diethyinitrosamine (DEN) in iodized oil by left intra-bronchial instillation into 80 Wistar rats. Another 10 Wistar rats receiving intrabronchial instillation of iodized oil served as control. In 15, 35, 55, 65 and 75 d after the instillation, 16 rats from experimental group and 2 from the control were sacrificed for HE and SP sta