单分子实时测序和其在微生物表观遗传学中应用.PDFVIP

微生物学通报 Jan. 20, 2017, 44(1): 186−199 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.160229 专论与综述 单分子实时测序及其在微生物表观遗传学中的应用 * 葛元洁 陈实 ( 430072) 摘 要：表观遗传学对于微生物的生命进程起着重要作用。由限制-修饰系统调控的DNA 修饰参 与微生物的免疫防御系统，无限制-修饰系统调控的DNA 修饰通过调控基因表达影响表型。然而， 表观遗传信息还没有被常规地作为DNA 信息收集分析。基于对DNA 合成反应的动力学分析， 单分子实时测序技术可以在获得基本序列数据的同时实现对被修饰核苷酸的检测。这个技术为微 生物中已知DNA 修饰的研究提供了新的平台，也为新型DNA 修饰的发现做好准备。本文综述 了单分子实时测序技术及其在微生物表观遗传学中的应用。 关键词：单分子实时测序，表观遗传学，DNA 修饰 Single molecule real time sequencing and its applications in microbial epigenetics—a review * GE Yuan-Jie CHEN Shi (School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei 430072, China) Abstract: Epigenetics plays an important role in biological processes of microorganisms. DNA modification with restriction-modification system is a component of microbial immune system, and DNA modification without restriction system influences phenotypes by gene regulation. However, epigenetic information has not been collected for DNA sequence analysis routinely. Single molecule real time (SMRT) sequencing, based on analysis of DNA synthesis kinetics, enables the acquisition of DNA primary sequences and detection of modified nucleotides simultaneously. This technology provides a new platform for research on known DNA modifications and discovery of novel DNA modi