急性心肌缺血大鼠小肠孤啡肽OFQ表达的变化.doc

PAGE PAGE 2 冠脉结扎大鼠小肠孤啡肽表达的变化[ [基金项目]：国家自然科学基金资助项目30772083） [作者简介]：姚太平，麻醉学硕士，讲师，主治医师，主要从事麻醉与器官保护研究 [作者单位] 032200山西汾阳 山西医科大学汾阳学院麻醉学教研室（姚太平）；030001山西太原 山西医科大学第二医院麻醉科(郭政) [通讯作者]：郭政,Email:ykdgz2004@ 姚太平，郭政 【摘要】目的 观察结扎冠状动脉左前降支造成心肌缺血时大鼠小肠内孤啡肽的表达变化。方法 54只雄性SD大鼠按随机数字表法分分为三组:（1）空白对照组(Control group, C组)，麻醉后直接取材；（2）假手术组(Sham group, S组)，只开胸暴露心脏不结扎冠脉；（3）结扎冠状动脉组（Coronary artery occlusion group, CAO组），单纯扎闭冠状动脉左前降支。其中，Sham组和CAO组又分为四个时点的亚组：0.5h组、1h组、3h组、6h组，每组大鼠均为6只。采用免疫组化观察并分析结扎大鼠冠状动脉不同时点小肠组织内OFQ的表达变化情况；应用酶联免疫吸附技术对比分析不同时点小肠组织内OFQ的表达水平。结果 免疫组化切片以平均光密度为指标进行半定量分析。统计分析结果显示：CAO后各时点小肠粘膜和肌间神经丛OFQ的水平较对照组显著升高(P﹤0.05)，酶联免疫吸附技术结果显示CAO组小肠OFQ的水平较sham组、对照组显著升高(P﹤0.05)，CAO3h组表达最显著(P﹤0.01)；sham组与对照组之间比较无显著性差异(P﹥0.05)。结论 急性心肌缺血时大鼠小肠内孤啡肽的表达增加,它可能作为一种肠神经递质或调质，通过与其他肠神经递质相互的作用来影响胃肠运动和分泌。 【关键词】心肌缺血；小肠；孤啡肽 Study on the change of Orphanin FQ in intestine after coronary artery occlusion in the rats YAO Tai-ping，Fenyang college of Shanxi Medical University, Fen yang, 032200； GUO Zheng，Departments of Anesthesia of Second Hospital of Shanxi Medical University, Taiyuan, 030001 【Abstract】Objective：To observe the Orphanin FQ（OFQ) expression in rat intestine tissue of acute myocardial ischemia. Methods：260-280g healthy adult male SD rats were randomly divided into three groups (n = 54)：(1) animal control group；(2) Sham group；(3) coronary artery occlusion group. Using immunohistochemical staining (SABC) and Elisa technology to observe the expression of OFQ in the intestine tissue after coronary artery occlusion in different points in time. Results：The level of OFQ in CAO group were significantly higher than the control group (P 0.05), and reached its peak (P0.05) in the CAO 3h.Conclusions：Expression of OFQ significantly increased in rat intestine tissue of coronary artery occlusion groups.It may be as a bowel neurotransmitter or conditioning, through other intestinal neurotransmitter mutual effect to affect the movement and.secretion of gastric bowel . 【Key words】 Myocardial ischemia； Orphanin FQ；in