三聚氰胺表面等离子体共振免疫传感四检测研究-食品工程专业毕业论文.docxVIP

ABSTRACTIn ABSTRACT In recent years，me prob】em of mel撇ine illegally added into the foods and feeds is repeated，、vhich haS threatened to hulnan and pets health and safety．’11lus，a rapid，sensitiVe aIld stable surfke plaSmon resonallce(SPR)imm眦osensorbased on the i11llibition fomat was developed and Validated f研detec妇g melamine(MEL)in milk products and pet foods in this project． In也is experiment，MEL waS detected t11rough the challge of s啪ple response，which caused by the a11tigen—antibody binding reaction the chip surf．ace．In the optimized condition，t11e acetate buf．fer pH 4．5 was used to be coupling buffer for coating the chip． And O．1 mol L‘1 of HCl was selected to be me regeneration with a positive result，Which made sensor chips could be reused up to 500 times in montll with response Value Var)，ing less thaIl 5％．Di行erent concentrations of MEL、vere detected using 5“g mL．1锄tibody concemration谢m good liner mge(1．4×10-1一O．17斗g mL吐)in the method．And也e sensitivity(IC50)a11d t11e limit(IC 5)of detection of the proposed method for MEL、vere 2．32×1 0．2“g mL_and 1．4×1 0-j pg mL～respectiVely．The immunosensor was highly specific to MEL，wllich oIlly displayed 10w cross—reactiVity(ClU(0．0 1％)for cy锄uric acid，cyanuric c11loride aJld atrazine exc印t cyromazine．The aLssay was Validated for t11e detectioIi of MEL in氨Jll cre锄milk，skim milk powder’in‰t fo】仰ula，dog food a11d eat food嘶th a good recoVery results of 76．4％一1 05．0％；97．9％一99．9％；79．0-11 3．5％； 86．O％一113．3％；73．5％-109．4％espectiVely．The sensitiVity of the assay in each type of sanlple was 2．57×10。2 Ug mL～，2．32×lO’2 pg 91，2．51×10’2¨g g一，2．66×10’2“g g‘1 and 2．68×1 0～pg g～，reSpectiVely，which were much lower thaIl t11e maxim岫residue leVels(MRLs)ofMEL． By tllis me廿lod，it 011ly takes witllin 1 5mimnes to detect the residue MEL in food s锄ples．Under me optimal experimental conditions，SPR immunosensor shows sensitiVe， rapid and sta．ble to detect the MEL in food samples． Keywords： Mel跗1i11e； ImmuIlosensor； S晌ce pl煳on resonaI】ce； Milk； Pet food