芪水煎对糖尿病肾病小鼠肝、肾组织细胞.PDFVIP

摇1070 环球中医药2017年10月第10卷第10期摇 Global Traditional Chinese Medicine,October2017,Vol郾10,No郾10 ·论著 · 芪水煎对糖尿病肾病小鼠肝、肾组织细胞 外基质调节因子表达的影响 刘晔摇 车念聪摇 杜宇琼摇 王金光摇 李文新 揖摘要铱摇 目的摇 基于肝肾同源理论,观察糖尿病肾病(kk鄄ay)小鼠后期谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶含量(aspartate transaminase,AST)变化以及芪水煎对肝、肾基质 金属蛋白酶鄄9(Matrix metalloe proteinas鄄9,MMP鄄9)及基质金属蛋白酶组织抑制因子鄄1(tissue inhibitor of metalloproteinase鄄1,TIMP鄄1)表达的影响。方法摇 采用kk鄄ay小鼠高糖高脂饮食4周建立糖尿病肾 病模型后,随机分为模型组、黄芪水组、通心络组,设立 C57BL/ 6J小鼠作为对照组。 予芪水煎及通 心络干预 12 周后,监测各组小鼠血 ALT、AST,采用 Western Blot、Realtime鄄PCR 检测肝、肾组织 MMP鄄9和TIMP鄄1蛋白含量及 mRNA 表达水平。 结果摇 与模型组比较,芪水煎组 AST 显著降低 (P0.01)、ALT呈下降趋势(P0.05),肝MMP鄄9蛋白及mRNA表达显著升高(P0.01),TIMP鄄1蛋 白显著降低(P0.01),mRNA表达降低(P0.05),肾MMP鄄9 蛋白及 mRNA表达升高(P0.05), TIMP鄄1蛋白及mRNA表达降低(P0.05)。 结论摇 kk鄄ay小鼠后期会出现肝损伤,芪水煎对kk鄄ay小 鼠肝、肾损伤具有一定的保护作用。 揖关键词铱摇 黄芪;摇 水红花子;摇 肝肾同源;摇 肝损伤;摇 肾基质金属蛋白酶鄄9;摇 基质金属蛋 白酶组织抑制因子鄄1 揖中图分类号铱摇 R285.5摇 揖文献标识码铱摇 A摇 doi:10郾3969/ j郾issn郾1674鄄1749郾2017郾10郾003 Effects of Qishui decoction on the expression of extracellular matrix regulatory factors in liver and kidney of diabetic nephropathy mice摇 LIU Ye,CHE Niancong,DU Yuqiong,et al.the Capital Medical University,traditional Chinese medicine ,Beijing 100069,China Corresponding author:Che Niancong,E鄄mail:cnc088@ 揖Abstract铱摇 Objective摇 To investigate the content changes of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) of diabetic nephropathy (kk鄄ay) mice in later stage,and to investigate the influence of Qishui decoction on the expression of MMP鄄9,TIMP鄄1inthehepatic andnephritictissues based on the theory of “homogeny of liver and kidney冶. Methods摇 The diabetic nephropathy model was establishedby kk鄄ay micewithhighglucoseandhighfat dietfor4 weeks. Themicewererandomly divided into model group,Qishui group and Tongxinluo group,and C57BL/ 6J mice were used as control group. After 12 weeks of drug intervention,t