厚朴酚对大鼠急性坏死性胰腺炎急性肺损伤的保护作用-中医内科临床专业论文.docxVIP

Y Y 1001599 成都中医药大学硕士学位论文 中文摘要 目的：观察中药提取物厚朴酚(Mag)对大鼠急性坏死性胰腺炎(ANP) 肺组织中核因子一KB(NF-KB)及血清中肿瘤坏死因子(TNF一Ⅸ)和白 介素一6(IL一6)表达的影响，以探讨厚朴酚对ANP急性肺损伤的保 护作用。 方法：以5％牛磺胆酸钠逆行胆胰管注射制备大鼠ANP模型，56只SD 大鼠随机分为假手术(SO)组，ANP模型组，及Mag治疗组。以荧光定 量PCR检测肺组织中NF-KB的基因表达，同时检测各组血清淀粉酶 (Amy)、TNF—q和IL-6的水平，并观察胰腺和肺的组织病理学改交。 结果：与S0组相比，ANP组肺组织中的NF—I(B mRNA表达量及血清 Amy、TNF一Ⅸ和IL一6显著增加(PO。01)，其中尤以12h点的升高最 明显(P0．01)。与ANP各组比较，Mag各组NF—KB mRNA、TNF—a、IL一6 及Amy表达量F降，也以12h下降最显著(分别为L 30+0．14 VS 1．844-0．56，P0．05：43．154-5．93 VS 76．18+8．21，P0．01； 40．734-8．21 vS 65．784-5．06，P0．01： 6273．334-1492．87 VS 11315．004-1424．53，P0．01)。Mag治疗组肺组织病理学改变较ANP 组明显减轻，胰腺组织学损害无明显改善。 结论：NF—KB、TNF一仪和IL一6是ANP发生急性肺损伤的重要炎症因子， Mag能抑制其在ANP大鼠肺组织中的表达，对ANP合并急性肺损伤有 一定的治疗作用。 【关键词】急性坏死性胰腺炎；厚朴酚：急性肺损伤； 大鼠 盛塑主墨塑查兰堡主兰垡堕苎Abstract 盛塑主墨塑查兰堡主兰垡堕苎 Abstract Obj ect i ve-Magnolol(Mag)is a Chinese herb that has potent anti．inflammatory effects．Our aims were to investigate the effects of magnolol in the treatment of acute Itmg injurc and to assess its influence on the expression of tumor necrosis factor alpha(TNF-q)，interleukin fIL)．6 in blood serum and nuclear factor-KB(NF-K13)in lung tissue in acute necrotizing pancreatitis(ANP)in rats． Methods：ANP was induced by retrograde infusion of 5％sodium taurocholate into the biliary pancreatic duct of Sprague。Dawley rats． Fif【y．six rats were randomly divided into three groups：sham operation (SO)group，ANP 6 h，12 h，24h groups，and Mag 6 h，12 h，24h groups． The gene expression of NF·rd3 in lung tissue were assayed using fluorescence quantitative polymerase chain reaction(FQ PCR)．The activity of serum amylase(Amy)，TNF一仪and IL-6 was determined． Pathological changes ofthe pancreas and lung were observed． Resu I ts：Compared with that in SO group，The gene expression of NF．rd3，TNF一仪，IL一6 and serum Amy in ANP group were increased greatly(p0．o 1)，．Mag treatment resulted in lower gene expression of NF．rd3，n吓一Ⅸ，IL一6 and serum Amy levels(1．30士-0．1 4 VS 1．84+0．56， PO．05：43．15i5．93 VS 76．18士8．21，P0．01；40．73+8．21 VS 65．784-5．06， PO．Ol；6273．33