荆芥连翘汤促愈兔小腿溃疡模型实验研究.PDFVIP

·1346 · 南方医科大学学报(J South Med Univ) 2006;26(9) 荆芥连翘汤促愈兔小腿溃疡模型的实验研究 1 1 2 2 2 2 1 2 兰海梅 ，刘学清 ，陈宝田 ，杨 柳 ，钱 江 ，赖梅生 ( 南方医科大学南方医院 皮肤科，中医科，广东 广州 510515) 摘要:目的 观察荆芥连翘汤浸泡治疗实验性兔小腿溃疡的疗效。方法 成年新西兰雄性大白兔9 只，随机分为荆芥连翘 汤组、生肌玉红汤组和生理盐水组，建立小腿慢性溃疡模型，3 组同时分别浸泡皮损部位。定期肉眼观察溃疡面积大 小、深浅、表面分泌物等。3 周后，取溃疡边缘组织制片，分别做常规病理、电镜、VEGF 和CD34 免疫组织化学方法标记 观察毛细血管表达情况，并进行血管数量及面积计数。结果 治疗过程中，肉眼观察3 组之间差异不显著，但3 周后常规 病理、电镜结果显示3 组之间有差异。免疫组化VEGF 和CD34 标记显示，实验组和阳性组之间无显著差异（P0.05 ）， 但2 组均与阴性组之间有显著差异(P0.01) 。结论 荆芥连翘汤和生肌愈红汤均在治疗兔小腿溃疡过程中有明显促进 溃疡愈合作用。 关键词：荆芥连翘汤；小腿溃疡；药效学 中图分类号：R289.5 文献标识码：A 文章编号：1673-4254 （2006 ）09-1346-04 JingjielianqiaoDecoctionpromoteslegulcerhealinginrabbits 1 1 2 2 2 2 LAN Hai-mei , LIU Xue-qing , CHEN Bao-tian , YANG Liu , QNG Jing , LAI Mei-sheng 1 2 Department of Dermatology , Department of Traditional Chinese Medicine , Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract:Objective To study the effect of Jingjielianqiao Decoction in promoting leg ulcer in rabbits. MethodsNine adult male New Zealand albino rabbits with chronic leg ulcers were randomized into 3 groups, namely group A treated with Jingjielianqiao Decoction, group B with Shengjiyuhong Decoction, and group C with normal saline. Gross observation of the wounds was carried out regularly for evaluating the changes in the ulcerous area, depth and wound surface excretion. After 3 weeks of treatment, the tissues on the edge of the ulcer were sampled and prepared for routine pathological examination, electron microscopy and immunohistochemistry for vascular endothelial growth factor (VEGF) and