上海市嘉定区生禽畜类食品中沙门氏菌污染情况及血清学研究.docx

上海市嘉定区生禽畜类食品中沙门氏菌污染情况及血清学研究 摘要：【目的】分析嘉定区2011―2014年生禽畜肉类沙门氏菌污染情况，为有针对性地制定生禽畜类食品防控措施提供科学依据。【方法】按照GB 4789.4―2010 《食品安全国家标准 食品微生物学检验沙门氏菌检验》检测沙门氏菌污染状况及其血清学分型。【结果】2011―2014年共检测样品600件， 检出阳性菌株154件， 总检出率为25.67%，鸡肉类总的阳性率为29.86%，鸭肉类为28.33%，猪肉类为27.78%，牛肉类为20.83%，羊肉类为17.71%。沙门氏菌优势血清型为肠炎沙门氏菌、鼠伤赛沙门氏菌、德尔卑沙门氏菌及印第安纳沙门氏菌。【结论】嘉定区生禽畜肉类食品存在不同程度上的食源性致病菌污染，沙门氏菌阳性检出率处于比较高的水平，应该加强对这类食品的监管，健全完善食品风险监测网络体系。 关键词：沙门氏菌；防控措施；食源性致病菌 中图分类号：R15 文献标志码：A DOI：10.19428/j.cnki.sjpm.2018.18418 引用格式：高付敏，陈培超，陈伟鑫，等.上海市嘉定区生禽畜类食品中沙门氏菌污染情况及血清学研究[J].上海预防医学，2018，30（9）：755-758. Abstract： [Objective] To analyze Salmonella contamination status of raw poultry and livestock meat in 2011-2014 in Jiading District， then providing theoretical basis for Salmonella detection，supervision，control and its safety management.The dominant species of bacteria are Salmonella enteritidis，S.typhimurium，Salmonella derby，Salmonella Indiana. [Methods] On the basis of criterion of national food sanitation examination method （GB 4789.4―2010） were determined Salmonella contamination and its serologic type. [Results] A total of 600 samples of chilled meat were examined from 2011 to 2014，and 154 strains of Salmonella were isolated and the total positive rate for all samples was 25.67%.The total positive rate for chickens was 29.86%.Chicken meat was the most seriously contaminated，followed by the chilled meat of duck，pork，beef and lamb，for 28.33%，27.78%，20.83% and 17.71%，respectively. [Conclusion] The chilled meat of poultry and livestock in Jiading District has been contaminated by Salmonella in different degrees，surveillance should be enhanced to ensure food safety and food risk monitoring network system is improved and perfected. Keywords：Salmonella；prevention and control measure；foodborne pathogen 沙氏菌在自然界中分布广泛，主要存在于动物肠道中，禽类、生猪及其鲜肉制品的感染率均很高，沙门氏菌是引起食源性疾病（包括食物中毒）主要的致病菌，以沙门氏菌引起的食物中毒占细菌性食物中毒的首位[1-2]。防控沙门氏菌污染是预防食源性疾病的关键。本文通过对嘉定区2011―2014年生禽畜类食品中沙门氏菌监测资料的分析，了解生禽畜类食品中沙门氏菌污染状况和分子特征，确定分离菌株的血清型及优势血清型分布，掌握沙门氏菌污染的动态变化，为制定生禽畜类食品安全措施提供科学依据。 1 材料与方法 1.1 样本