基因转染对下颌骨牵引区细胞生长因子表达的影响-外科学专业论文.docxVIP

泸州院学院硕士研究生学位论文 泸州院学院硕士研究生学位论文 PAGE PAGE 13 量的生理盐水。 5 组实验动物均施加电穿孔刺激。各组分别于固定期第 7，14、28 天行常气栓塞处死动物，切取牵引区新生骨组织，进行组织学检查，免搜细化染色 检测新生骨中的 BMP、VEGF ，FGF，TGF币、 CyclinsA 、Dl、巴的表达情况。采用 图像分析系统分析。 结果: 免疫组化染色显示 BMP、VEGF ，FGF、TGF币、 CyclinsA 、Dl、E 要在肉芽组织中的炎细胞如单核细胞、成纤维细胞及少量沿牵张方向排列的新生幼 稚骨小梁表丽的成骨细胞、骨细胞和骨周围结缔组织中表达。这些生长肉子均在固 定?天时表达最强烈， 14 天时减弱， 28 天时部分生长因子仅微弱表达成阴性表达。 图像分析结果显示慕因治疗组 (A、日、C 组)的生长因子表达在各时期均明显高于 对照组 (D、E 组)。 结论z 在∞过程中，电穿孔介导的基因泊疗能旬效地促进多种细胞因子的表 达，这些细胞因子的高表达对Jfn.管生戚、细胞的分化与增殖功能起着草耍的作用， 从而刺激牵引区骨基质含成和诱导成纤维细胞、成骨细胞，血管内皮细胞、骨细胞 的增硝分化等活性功能，促使早期微血管做成，更能有效的促进骨的生长和修复。 这可能是基因治疗促进牵引间隙新骨生成的分子机理。 提键词:电穿孔:基因治疗:牵引成骨:下顿骨:细胞因子;免疫组化 泸州阪学院硕士研究 泸州阪学院硕士研究牛引以论文 Effects of Geoe Traosfection 00 the Expression of Celluer Growth Factor in Mandible Distraction area ABSTRACT Background: Distraction osteogenes，which is an im阴阳nt endogenous bone tissue engineering，provide an important method for correction the congenital ，acquired deformities and defects. Meanwhile ，distraction osteogensis have relatively long time， probably lead ωsome complications and social-mental problems ，which remain to be further resolved. On the other hand ，the mechanisms of how the distraction mechanical tension convert to biological signal is still unclear to us. Therefore，how to promote new bone formation and regulate the cellular factors during distraction became one of hot topics at present.In the perious s阳dy，we have established electroporatior←mediated gene therapy animal models of rabbit mandibular distraction osteogenesis successfully，and the studies suggest th创 the electroporation-mediated gene therapy can promote angiogenesis and calcium deposition during early stage of mandible DO，strengthen the bone mineral density and s旺ness of new formed bone. Based on those studies，伽e present s阳dy is ω further investigate the e仔ect on cytokine expression in the distraction area a白er electroporation-mediated gene therapy. The aim of this study isωexplore the mechanism of it promote new bone formation. Objectiv