基于“钙信号通路-血脑屏障”偶联的中药脑神经保护有效组分筛选方法研究-中药学-中药药效和毒理专业论文.docxVIP

成都中医药大学博士学位论文 成都中医药大学博士学位论文 4 4 Chuanxiong aetherolea,chuanxiongzine,ferulaic acid and shichangpu aetherolea.Both scutellarin and asaricin could only resist cortical neurons cell injury caused by glutamate. The blood-brain barrier model in vitro was successful. erigeron injection， 3,5-di-O-caffeoyl quinic acid，aetherolea from Chuanxiong，chuanxiongzine，ferulaic acid, aetherolea from Shichangpu and asaricin could penetrate BBB, but scutellarin didn’t,the products of the structural modification of which, scutellarin ethyl ester, meanwhile，has neuroprotective effect and regulation of calcium signal pathway . 3,5-di-O-caffeoyl quinic acid and aetherolea from Chuanxiong Chuanxiong could enhance the activities of SOD, GSH-Px ,NOS and decrease the content of MDA in rats to resist the damages caused by ischemical reperfusion injury . Aggregate analysis indicated that the neuroprotective effect of erigeron injection is related to regulation of calcium signal pathway ,so did 3,5-di-O-caffeoyl quinic acid ， aetherolea from Chuanxiong ， chuanxiongzine ， aetherolea from Shichangpu and asaricin. Furthermore, the ability to penetrate BBB of active ingredients is an important factor to resist cerebral ischemia. Conclusions: All the results above showed can elementarily attest to that establishing a screening method of cerebral nerves protective composition from TCM based on “calcium signal pathway - blood brain barrier”is feasible，and offered a new research idea for development of original new drug to treat cerebral ischemia. Keywords:Calcium signal pathway；Blood-brain barrier；Neuroprotective；Cerebral ischemia；Erigeron breviscapus；Chuanxiong；Shichangpu；Active ingredients PAGE PAGE 7 英文缩写词对照表 ICVD ischemia cerebral vascular disease 缺血性脑血管疾病 PCD programmed cell death 程序性细胞死亡 AC Astrocytes cells 星型胶质细胞 BBB Blood-brain barrier 血脑屏障 BBBM Blood-brain barrier model 血脑屏障模型 BMVEC Brain microvascular endothelial cells 脑毛细血管内皮细胞 BSA Bovine serum albuminnn 牛血清白蛋白 b-FGF Basic fibroblast g