严格厌氧发酵体系生产丁醇及在线尾气监测系统的设计-但生物学专业毕业论文.docxVIP

严格厌氧发酵体系生产丁醇及在线尾气监测系统的设计高19．O％，并且丁醇丙酮比相对于对照组的2．45提高了0．36。游离发酵的发酵周期为 严格厌氧发酵体系生产丁醇及在线尾气监测系统的设计 高19．O％，并且丁醇丙酮比相对于对照组的2．45提高了0．36。游离发酵的发酵周期为 44 h，固定化发酵周期明显缩短了，丁醇的生产强度显著提高。固定化发酵周期为32 h， 丁醇最高产量为15．4 g／L，生产强度0．64 g／(L·ho)，分别比对照组提高了12．4％和106％， C02和H2最高产气速率分别提高了60％和9％，产气量分别提高了20．7％和41．3％。加 入中性红的固定化发酵，丁醇和ABE的最高产量为16．8 g／L和26．8 g／L，丁醇产量和 ABE产量分别提高了22．6％和25．8％。 用酸水解和酶水解的方法水解海带，我们可以建立海带生产甘露醇和还原糖的最佳 条件。最佳的酸解条件为底物浓度为8％、酸解时间为90 min、酸解温度为120℃、酸 浓度为0．5 M。对酸解后的水解液进行酶解，甘露醇和还原糖的含量都有所提高。以海 带水解液为底物发酵产丁醇，总糖23 g／L，丁醇产量为1．2 g／L，得率为0．05 g／g 关键词：丁醇；厌氧发酵；尾气在线监测；海带 万方数据 大连理工大学硕士学位论文Butanol 大连理工大学硕士学位论文 Butanol Production in Anaerobic Fermentation System and The Design of Online Off-gas Analysis for Anaerobic Fermentation Abstract Butanol，is not only an important organic chemical raw materials，but also a new biofuel with great potential．However，using the conventional acetone-butanol-ethanol(ABE) fermentation to produce bio-butanol has some questions．Due to the presence of toxic of butanol inhibition，it will result in final low product concentration and low productivity．In addition，the high cost ofraw materials is another factor in the development ofbio-butan01． Then online off-gas analysis for anaerobic fermentation can be desinged．Using the online off-gas analysis，the fermentation performance of Cacetobutylicum were investigated． The online off-gas analysis system can provide detailed gas datas to analysis anaerobic metabolic process。 Adding neutral red and using immobilization technology can produce a high concentration and high productivity butan01．Seaweed，is raw materials。A method of pretreatment and enzymatic saccharification technology has been established．Then we can explore the content of reducing sugars and mannitol in seaweed，to conduct feasible study of its energy． The design of anaerobic fermentation off-gas online system is feasible．The volume of oxygen in this system is only 5．9 mL，the volume of oxygen in aerobic fermentation off-gas online system is 1 23．7 mL．The aerobic fermenta