DNA固定在纳米颗粒表面的方法.ppt

磷酸化的DNA Biotin-DNA 炔基修饰的DNA 巯基修饰的DNA 氨基修饰的DNA 金银纳米颗粒 碳纳米管、石墨烯 二氧化硅纳米颗粒 量子点 上转换纳米颗粒 磷酸化的DNA与TiO2 π-π堆积，如单链DNA与石墨烯，碳纳米管 静电吸附 1、Biotin-DNA 2、炔基修饰的DNA及其他生物分子 3、NH2-DNA 氨基 羧基 氨基 羧基 氨基 氨基 1）EDC和NHS For best results in two-step reactions, perform the first reaction in MES buffer (or other non-amine, non-carboxylate buffer) at pH 5-6, then raise the pH to 7.2-7.5 with phosphate buffer (or other non-amine buffer) immediately before reaction to the amine-containing molecule 氨基 羧基 DCC（N,N′-Dicyclohexylcarbodiimide） 氨基-氨基 1）BS(PEG)n PEG Crosslinkers - Amine Reactive 2）TSAT (Tris-succinimidyl aminotriacetate) Water Water NHS esters:pH 7-9 3）DSS (disuccinimidyl suberate) 4）BSOCOES (bis[2-(succinimidyloxycarbonyloxy)ethyl]sulfone) 5）DSG (disuccinimidyl glutarate) 6）DTSSP (3,3′-Dithiobis[sulfosuccinimidylpropionate]) Water Water Water DMSO or DMF 8）BS3 (Bis[sulfosuccinimidyl] suberate) 9）EGS [ethylene glycolbis(succinimidylsuccinate)] 10）Sulfo-EGS [ethylene glycolbis(sulfosuccinimidylsuccinate)] 7）DST (disuccinimidyl tartrate) Water Water DMSO or DMF DMSO or DMF 4、巯基化的DNA 1 Au-S Ag-S 2 巯基 氨基 3 巯基 羧基 4 巯基 羟基 5 巯基 巯基 巯基-氨基 1）AMAS (N-[α-maleimidoacetoxy] succinimide ester) NHS esters:pH 7-9；Maleimides:pH 6.5-7.5；Usually 7.2-7.5。Two steps reaction DMSO or DMF 2）BMPS (N-[β-maleimidopropyloxy] succinimide ester) DMSO or DMF 3）EMCS (N-[ε-maleimidocaproyloxy]succinimide ester) DMSO or DMF 4）Sulfo-EMCS (N-[ε-maleimidocaproyloxy]sulfosuccinimide ester) Water 6）Sulfo-GMBS (N-[γ-maleimidobutyryloxy]sulfosuccinimide ester) 5）GMBS (N-[γ-maleimidobutyryloxy]succinimide ester) DMSO or DMF Water 7）BMPA (N-β-maleimidopropionic acid) EDC，0.1mol MES pH4.7 Maleimides react with sulfhydryls (–SH) at pH 6.5-7.5 8）LC-SMCC (Succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxy-[6-amidocaproate]) DMSO or DMF 10）Sulfo-SMPB (sulfosuccinimidyl 4-[p-maleimidophenyl]butyrate) 9）SMPB (succinimidyl 4-[p-maleimidophenyl]butyrate) DMSO or DMF Water 11）Sulfo-LC-SPDP, LC-SPDP, SPDP 巯基-羧基 巯基-羟基 异氰酸脂在水溶液中很容易水解