一种适合转基因棉CpTI和cry1A基因剂量测定的标准质粒的-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2011, 37(9): 1533−1539 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2011.01533 一种适合转基因棉CpTI 和cry1A 基因剂量测定的标准质粒的构建和应用 苏长青1,2 谢家建1,* 孙 爻1 彭于发1,* 1 / () / , 100193; 2 , 053000 : , , (Gossypium hirsutum L.), (CpTI)(cry1A) - (Sad1)pMD-CCS , CpTI cry1A PCR 9 CpTI cry1A , 3 3 CpTI 0.020~0.018 /, cry1A 1.377~2.136 /; 1 F1 6 cry1A 0.887~2.564 /, (SD)0.001~0.149 , pMD-CCS CpTI cry1A : ; CpTI ; cry1A ; PCR; Construction and Application of a Reference Plasmid Suitable for Determina- tion of CpTI and cry1A Gene Dosages in Genetically Modified Cottons 1,2 1,* 1 1,* SU Chang-Qing , XIE Jia-Jian , SUN Yao , and PENG Yu-Fa 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests / Institute of Plant Protection, China Inspection Test Center for Environmental Safety of Transgenic Crops, Ministry of Agriculture / Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2 Department of Life Sci- ence, Hengshui College in Hebei, Hengshui 053000, China Abstract: In transgenic detection field, a preferable reference material (RM) has been developing with the advantage of easy availability, high purity, low cost and good stability, which is more suitable for detecting multiple target genes. In the present study, we constructed a multi-target reference pla